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Characterization of Electron Transfer Dissociation in the Orbitrap Velos HCD Cell

  • Focus: Electron Transfer Dissociation: Research Article
  • Published:
Journal of The American Society for Mass Spectrometry

Abstract

Electron transfer dissociation (ETD) is commonly employed in ion traps utilizing rf fields that facilitate efficient electron transfer reactions. Here, we explore performing ETD in the HCD collision cell on an Orbitrap Velos instrument by applying a static DC gradient axially to the rods. This gradient enables simultaneous three dimensional, charge sign independent, trapping of cations and anions, initiating electron transfer reactions in the center of the HCD cell where oppositely charged ions clouds overlap. Here, we evaluate this mode of operation for a number of tryptic peptide populations and the top-down sequence analysis of ubiquitin. Our preliminary data show that performing ETD in the HCD cell provides similar fragmentation as ion trap-ETD but requires further optimization to match performance of ion trap-ETD.

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Acknowledgments

The Netherlands Proteomics Centre, a program embedded in The Netherlands Genomics Initiative, is kindly acknowledged for financial support. Part of this research was performed within the framework of CTMM, the Center for Translational Molecular Medicine (www.ctmm.nl), project CIRCULATING CELLS (grant 01C-102).

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Author notes

  1. Shabaz Mohammed

    Present address: Departments of Chemistry and Biochemistry, University of Oxford, Physical & Theoretical Chemistry Laboratory, South Parks Road, OX1 3QZ, Oxford, UK

Authors and Affiliations

  1. Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CH, Utrecht, The Netherlands

    Christian K. Frese, A. F. Maarten Altelaar, Shabaz Mohammed & Albert J. R. Heck

  2. Netherlands Proteomics Centre, 3584 CH, Utrecht, The Netherlands

    Christian K. Frese, A. F. Maarten Altelaar, Shabaz Mohammed & Albert J. R. Heck

  3. Thermo Fisher Scientific, 28199, Bremen, Germany

    Dirk Nolting & Jens Griep-Raming

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  1. Christian K. Frese
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  2. Dirk Nolting
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  3. A. F. Maarten Altelaar
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Correspondence to Shabaz Mohammed or Albert J. R. Heck.

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Frese, C.K., Nolting, D., Altelaar, A.F.M. et al. Characterization of Electron Transfer Dissociation in the Orbitrap Velos HCD Cell. J. Am. Soc. Mass Spectrom. 24, 1663–1670 (2013). https://doi.org/10.1007/s13361-013-0618-9

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  • DOI: https://doi.org/10.1007/s13361-013-0618-9

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