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Carbonation and Other Super Saturated Gases as Solution Modifiers for Improved Sensitivity in Solvent Assisted Ionization Inlet (SAII) and ESI

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Journal of The American Society for Mass Spectrometry

Abstract

Solvent Assisted Ionization Inlet (SAII) produces ions in a heated inlet to a mass spectrometer from aqueous and aqueous/organic solutions with high sensitivity. However, the use of acid modifiers, which typically aids electrospray ionization, generally results in ion suppression in SAII. Here we demonstrate that the use of carbonation and other super-saturated gases as solution modifiers increases analyte ion abundance and reduces metal cation adduction in SAII. Carbonation is also found to enhance electrospray ionization. The mechanistic and practical utility of carbonation in mass spectrometry is addressed.

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Acknowledgments

This work was made possible by funding from NSF CHE-1112289 and the Richard Houghton endowment from the University of the Sciences.

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Authors and Affiliations

  1. University of the Sciences in Philadelphia, Philadelphia, PA, 19104, USA

    Vincent S. Pagnotti, Shubhashis Chakrabarty & Charles N. McEwen

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  1. Vincent S. Pagnotti
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  2. Shubhashis Chakrabarty
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  3. Charles N. McEwen
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Correspondence to Charles N. McEwen.

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Vincent S. Pagnotti and Shubhashis Chakrabarty contributed equally.

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Pagnotti, V.S., Chakrabarty, S. & McEwen, C.N. Carbonation and Other Super Saturated Gases as Solution Modifiers for Improved Sensitivity in Solvent Assisted Ionization Inlet (SAII) and ESI. J. Am. Soc. Mass Spectrom. 24, 186–192 (2013). https://doi.org/10.1007/s13361-012-0535-3

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  • DOI: https://doi.org/10.1007/s13361-012-0535-3

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