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Existence of two strains of Habrobracon hebetor (Hymenoptera: Braconidae): a complex in Thailand and Japan

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Abstract

Habrobracon hebetor Say (Hymenoptera: Braconidae) is a cosmopolitan gregarious ectoparasitoid that attacks larvae of several species of Lepidoptera. Although there are two genetically different strains within H. hebetor, distribution of the strains has been poorly understood. In 2010, in Thailand, where H. hebetor has been known as a parasitoid of stored grain pests, it was found that H. hebetor attacked Opisina arenosella Walker (Lepidoptera: Oecophoridae), which is an invasive pest of coconut palm. For correct identification of this H. hebetor, we conducted DNA analysis and cross tests using populations collected from O. arenosella and stored grain pests in Thailand and populations in Japan known as H. hebetor. We obtained 413 bp of mitochondrial cytochrome oxidase I (COI) sequences and 414 bp of 16S rRNA gene sequences, and both indicated that there are two distinct clades within H. hebetor: one contains insects from Thailand, Spain, India, and Barbados; the other contains insects from Japan and the USA. There were no genetic differences or sexual isolation between Thai populations from different hosts. Our results also showed that populations in Thailand were sexually isolated from a H. hebetor population in Japan.

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Acknowledgments

We thank Associate Prof. Dr. Anuchit Chinajariyawong and Prof. Dr. Kaoru Maeto for valuable comments on an earlier version of the manuscript. We thank Dr. Akihiro Miyanoshita for providing the Plodia interpunctella used in this study. This study was supported by the Research and Researchers for Industries (RRI), Ampol Food Processing, Ltd., and The Thailand Research Fund (TRF) (PHD56I0075 Code 56-106-0085).

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Authors and Affiliations

  1. Department of Entomology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand

    Namphueng Chomphukhiao & Sopon Uraichuen

  2. Faculty of Agriculture, Kyushu University, 744 Motooka, Fukuoka, 819-0395, Japan

    Shun-ichiro Takano & Keiji Takasu

  3. National Biological Control Research Center-Central Regional Center, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, 73140, Thailand

    Sopon Uraichuen

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  1. Namphueng Chomphukhiao
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  2. Shun-ichiro Takano
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  3. Keiji Takasu
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  4. Sopon Uraichuen
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Correspondence to Sopon Uraichuen.

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Chomphukhiao, N., Takano, Si., Takasu, K. et al. Existence of two strains of Habrobracon hebetor (Hymenoptera: Braconidae): a complex in Thailand and Japan. Appl Entomol Zool 53, 373–380 (2018). https://doi.org/10.1007/s13355-018-0568-6

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  • DOI: https://doi.org/10.1007/s13355-018-0568-6

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