Abstract
A polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP)-based method for species identification was applied to seven Japanese Lymantria species, including four Asian gypsy moth (AGM) species. We sequenced the partial end of the cytochrome c oxidase I (COI) gene, tRNA leucine, COII gene, and partial end of the tRNA lysine in mitochondrial DNA (mtDNA) for one individual of each of the seven species. We analyzed the recognition sites of three restriction endonucleases and constructed a scheme for Lymantria species identification using PCR–RFLP. We then applied the scheme to 291 individuals from 45 populations of seven species. We found that all seven species were correctly identified using PCR–RFLP. These results suggest that PCR–RFLP is useful for identifying Japanese Lymantria species, which may be detected at Japanese ports.
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Acknowledgments
We thank Prof. Y. Higashiura, Tokyo University of Pharmacy and Life Sciences, Dr. S. Yoshimatsu, National Institute for Agro-Environmental Sciences, and Mr. K. Arakawa, Mr. M. Satoh, and Dr. S. Takano, Yokohama Plant Protection Station who provided useful suggestions. We also thank Dr. K. Matsumoto, Tohoku Research Center, Forestry and Forest Products Research Institute who provided specimens of L. mathura collected at Morioka city, Iwate Prefecture. The remaining Lymantria specimens analyzed in the present study were collected by plant quarantine officers in several Plant Protection Stations.
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Arimoto, M., Iwaizumi, R. Identification of Japanese Lymantria species (Lepidoptera: Lymantriidae) based on PCR–RFLP analysis of mitochondrial DNA. Appl Entomol Zool 49, 159–169 (2014). https://doi.org/10.1007/s13355-013-0235-x
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DOI: https://doi.org/10.1007/s13355-013-0235-x