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Development of a multiplex PCR assay for the identification of eight species members of the Thai Hyrcanus Group (Diptera: Culicidae)

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Abstract

The Hyrcanus Group comprises important vectors of malaria because of Plasmodium vivax Grassi and Feletti and filariasis caused by Brugia malayi Brug in many countries of South, Southeast and East Asian regions. In Thailand, eight species members (Anopheles argyropus Swellengrebel, An. crawfordi Reid, An. nigerrimus Giles, An. nitidus Harrison, Scanlon & Reid, An. paraliae Sandosham, An. peditaeniatus Leicester, An. pursati Laveran and An. sinensis Wiedemann) of the Hyrcanus Group have been recognized. Due to morphological overlap, adult females of the Hyrcanus Group in Thailand have been misidentified markedly among the eight species, particularly when using the traumatic scales of wild-caught specimens from epidemiology and control approaches. Therefore, this study first developed a simple and robust multiplex PCR assay, based on second internal transcribed spacer sequences of ribosomal DNA, for differentiating the eight species members of the Thai Hyrcanus Group.

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Acknowledgments

This work was supported by the Thailand Research Fund (TRF Senior Research Scholar: RTA5480006) and the Diamond Research Grant of the Faculty of Medicine, Chiang Mai University, to W. Choochote and A. Saeung. The authors would like to thank Dr. Wattana Navacharoen, Dean of the Faculty of Medicine, Chiang Mai University, for his interest in this research.

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Authors and Affiliations

  1. Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand

    Chayanit Hempolchom, Atiporn Saeung, Kritsana Taai, Pradya Somboon & Wej Choochote

  2. Department of Infectious Disease Control, Faculty of Medicine, Oita University, Oita, 879-5593, Japan

    Yasushi Otsuka

  3. Department of Biology and Centre for Vectors and Vector-Borne Diseases, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand

    Visut Baimai

  4. Faculty of Veterinary Science (Establishment Project), Prince of Songkla University, Songkhla, 90110, Thailand

    Sorawat Thongsahuan

  5. Entomology Section, Queen Sirikit Botanic Garden, P.O. Box 7, Chiang Mai, 50180, Thailand

    Wichai Srisuka

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  1. Chayanit Hempolchom
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  2. Yasushi Otsuka
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Correspondence to Wej Choochote.

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Hempolchom, C., Otsuka, Y., Baimai, V. et al. Development of a multiplex PCR assay for the identification of eight species members of the Thai Hyrcanus Group (Diptera: Culicidae). Appl Entomol Zool 48, 469–476 (2013). https://doi.org/10.1007/s13355-013-0207-1

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