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Treatment of cutaneous candidiasis through fluconazole encapsulated cubosomes

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Abstract

Cubosomes encapsulating fluconazole were prepared by emulsification method and characterized for particle size, entrapment efficiency, SEM, in vitro release, skin irritation studies, and confocal laser scanning microscopy. The cubosomes prepared were 257.2 ± 2.94 nm in size and with drug entrapment efficiency of 66.2 ± 2.69 %. The prepared formulation was characterized for surface morphology by SEM analysis which revealed their smooth surface. The cumulative percentage of fluconazole from cubosomes permeated via dialysis membrane (molecular weight cutoff (MWCO) 12–14 kD) showed 76.86 % cumulative drug release, while fluconazole solution showed release up to 91.04 % in 24 h in phosphate-buffered saline (PBS) (pH 6.5), and sustained release is obtained after 24 h in case of cubosomes. The animal studies also revealed that the cubosomes are non-irritant and have sustained antifungal activity.

Overview of fluconazole encapsulated cubosomes from preparation to its antifungal action

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Acknowledgments

All the authors are thankful to AIIMS, New Delhi, for SEM facility and RGPV, Bhopal, for DSC studies. One of the authors, Ms. Aakanchha Jain, wishes to acknowledge Dr. Shushant Shrivastava, BHU, Varanasi, for CLSM facilities and to INSPIRE DST for Ph.D. fellowship. One of the authors, Dr. Richa Jain, is thankful to SJPN, Peoples Group, Bhopal.

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All the authors declare no conflict of interest.

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Authors and Affiliations

  1. Biopharmaceutics Research Laboratory, Department of Pharmaceutical Sciences, Dr. H. S. Gour University, Sagar, 470 003, India

    Vijay Prajapati, Aakanchha Jain, Sanjeev Sahu & Dharm Veer Kohli

  2. Centre for Scientific Research and Development, People’s University, Bhopal, India

    Richa Jain

Authors
  1. Vijay Prajapati
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  2. Aakanchha Jain
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  3. Richa Jain
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  4. Sanjeev Sahu
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  5. Dharm Veer Kohli
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Correspondence to Dharm Veer Kohli.

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Prajapati, V., Jain, A., Jain, R. et al. Treatment of cutaneous candidiasis through fluconazole encapsulated cubosomes. Drug Deliv. and Transl. Res. 4, 400–408 (2014). https://doi.org/10.1007/s13346-014-0202-2

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  • DOI: https://doi.org/10.1007/s13346-014-0202-2

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