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Polycaprolactone scaffold as targeted drug delivery system and cell attachment scaffold for postsurgical care of limb salvage

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Abstract

In this paper, a dual-function drug-laden polycaprolactone scaffold, which can serve as both targeted drug delivery system and attachment platform for tissue regeneration for the postsurgical care of limb salvage procedure, was developed with a simple and solvent-free molding technique. Scaffolds of varying surface architecture were created using poly(ethylene glycol) diacrylate microneedle arrays. A model drug, rhodamine B, was incorporated homogenously into the scaffold. In vitro drug release studies showed that rhodamine B was released in a slow and sustained manner for 112 days. Its release rate was affected by drug loading and scaffold surface architecture. Release of rhodamine B from the scaffolds followed the Higuchi diffusion model. Other drugs, namely, doxorubicin and lidocaine hydrochloride, were also effectively loaded into and released from the scaffolds. Cell attachment study demonstrated potential for the scaffolds to provide attachment platforms for tissue regeneration.

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Acknowledgments

The authors would like to express his gratitude to Dr. Alexandre Chan and Dr. Richard Quek from the National Cancer Center Singapore for their comments and inputs. The authors also thank Jaspreet S. Kochhar for his assistance in microneedle fabrication.

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Authors and Affiliations

  1. Department of Pharmacy, National University of Singapore, 18 Science Drive 4, Singapore, 117543, Singapore

    Bin Sheng Wong & Lifeng Kang

  2. Division of Bioengineering, School of Chemical and Biomedical Engineering, Nanyang Technological University, 70, Nanyang Drive, Singapore, 637457, Singapore

    Swee-Hin Teoh

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  1. Bin Sheng Wong
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Correspondence to Lifeng Kang.

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Supplementary Figure

Drug loading and drug loading efficiency. (a) Microscopic and fluorescence images of rhodamine B loaded PCL scaffolds. Rhodamine B was uniformly distributed throughout the scaffold. (b) Drug loading efficiencies over different multiple molding attempts using a single microneedle array. Near 100 % drug loading efficiencies were observed. (JPEG 38 kb)

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Wong, B.S., Teoh, SH. & Kang, L. Polycaprolactone scaffold as targeted drug delivery system and cell attachment scaffold for postsurgical care of limb salvage. Drug Deliv. and Transl. Res. 2, 272–283 (2012). https://doi.org/10.1007/s13346-012-0096-9

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