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Sapovirus detection and quantification in fecal samples from allogeneic hematopoietic stem cell transplant recipients

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Abstract

Sapovirus are important agents of acute gastroenteritis (AGE) and they are associated with outbreaks and sporadic cases worldwide. They infect people of all ages, but mainly children, the elderly and immunocompromised individuals are affected. The aim of this study was investigate sapovirus and to determine viral loads in fecal samples from patient undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT). Fecal samples were submitted to extraction of the genetic material using a commercial kit, and RT-qPCR TaqMan was used for sapovirus screening and determination of viral loads, using a standard curve with serial dilutions of a recombinant plasmid. Positive samples were sequence by Sanger method. Sapovirus was detected in one patient, 5.3% (1/19). Viral excretion lasted for 16 days. Viral load varied from 1.73 × 106 to 8.97 × 106 GC/g. One of the positive samples was characterized as GI.1 genotype. This is the first study to determine sapovirus loads in samples from allo-HSCT and to identify GI.1 genotype in immunocompromised patients.

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Acknowledgments

We thank all the staff members from the bone marrow transplant unit of Hospital Araujo Jorge who collected the patients' samples. This study was supported by Grant of Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (MCTI/CNPq/Universal 2014). The funders had no role in study design, data collection, and analysis, decision to publish, or preparation of the manuscript.

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Author notes

  1. Thairiny Neres Silva and Nathânia Dábilla have contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Virology and Cell Culture, Instituto de Patologia Tropical E Saúde Pública, Universidade Federal de Goiás - UFG, Rua 235, s/n, sala 420, Setor Universitário, Goiânia, GO, 74605050, Brazil

    Thairiny Neres Silva, Nathânia Dábilla, Thaís Santos Corrêa & Menira Souza

  2. Hospital Araújo Jorge/Associação de Combate Ao Câncer Em Goiás, Goiânia, GO, Brazil

    Adriano de Moraes Arantes

Authors
  1. Thairiny Neres Silva
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  2. Nathânia Dábilla
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  3. Thaís Santos Corrêa
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  4. Adriano de Moraes Arantes
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  5. Menira Souza
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Correspondence to Menira Souza.

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Neres Silva, T., Dábilla, N., Santos Corrêa, T. et al. Sapovirus detection and quantification in fecal samples from allogeneic hematopoietic stem cell transplant recipients. VirusDis. 31, 374–377 (2020). https://doi.org/10.1007/s13337-020-00583-1

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  • DOI: https://doi.org/10.1007/s13337-020-00583-1

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