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Sequence and phylogenetic analysis of canine parvovirus-2 isolates in dogs revealed circulation of three subtypes in Nigeria

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Abstract

Canine parvovirus-2 (CPV-2) is the most common viral cause of gastroenteritis in puppies in Nigeria and worldwide. After its emergence in 1978, the wild-type CPV-2 was rapidly replaced by three antigenic subtypes CPV-2a, CPV-2b and CPV-2c. Subtype CPV-2a has been reported in Nigeria based on limited number of samples. As such, this study was carried out with 56 faecal samples and four CPV-2 vaccines using polymerase chain reaction and sequencing to have a better idea of CPV-2 subtypes circulating in dogs in Nigeria. 54 (96.4%) out of the 56 samples were positive for CPV-2 subtypes. CPV-2a is the predominant subtype followed by CPV-2b and CPV-2c. Also, co-existence of subtypes CPV-2a and CPV-2b; and CPV-2a and CPV-2c were found in some dogs. Sequence analysis revealed the occurrence of three mutations D413 N, N426D/E, and T440A. To our knowledge, this is the first report of the three subtypes of CPV-2 in Nigeria.

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Acknowledgements

This work was supported in part by the AFSCAN grant. The author(s) received no financial support for the authorship and/or publication of this article.

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Authors and Affiliations

  1. Department of Veterinary Microbiology, University of Ibadan, Ibadan, Nigeria

    O. A. Fagbohun

  2. Department of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria

    T. O. Omobowale

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  1. O. A. Fagbohun
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  2. T. O. Omobowale
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Correspondence to O. A. Fagbohun.

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Fagbohun, O.A., Omobowale, T.O. Sequence and phylogenetic analysis of canine parvovirus-2 isolates in dogs revealed circulation of three subtypes in Nigeria. VirusDis. 29, 411–415 (2018). https://doi.org/10.1007/s13337-018-0475-z

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  • DOI: https://doi.org/10.1007/s13337-018-0475-z

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