Abstract
Porcine parvovirus (PPV) is a DNA virus of the genus Parvovirus of family Parvoviridae. It is the causative agent of many disease problems in pigs such as maternal reproductive failure, stillbirth, mummification, embryonic or fetal death, infertility, abortion and neonatal death. A study was conducted to assess the incidence of the virus in pigs in Kerala State in South India. A total of 38 samples were collected from domestic and wild pigs from different districts of the State. Polymerase chain reaction targeting a 265 bp fragment of the NS1 gene of the virus was carried out. Of the samples tested, 2 (5.26 %) were found to be positive for PPV virus genome, one of which was from a wild pig. One of the positive samples was sequenced and the nucleotide sequence obtained was compared with other sequences of PPV from India and abroad. The results revealed that the sequence had very close similarity to PPV sequences previously reported from India and to that of Chinese isolates. This is the first report of the existence of PPV in domestic and wild pigs in Kerala, India. The study highlights the need to test for the presence of PPV in addition to other infectious agents in diagnosis of cases of reproductive disorders in pigs.
This is a preview of subscription content, log in via an institution to check access.
References
Cartwright SF, Huck RA. Viruses isolated in association with herd infertility abortions and stillbirths in pigs. Vet Rec. 1967;81:196–7.
Choi CS, Molitor TW, Joo HS, Gunther R. Pathogenicity of a skin isolate of porcine parvovirus in swine fetuses. Vet Microbiol. 1987;15:19–29.
Hao X, Lu Z, Sun P, Fu Y, Cao Y, Li P, Bai X, Bao H, Xie B, Chen Y, Li D, Liu Z. Phylogenetic analysis of porcine parvoviruses from swine samples in China. Virol J. 2011;8:320–7.
Jeoung H, Lim S, Kim J, Cho Y, Kim YK, Song J, Hyun B, An D. Serological prevalence of viral agents that induce reproductive failure in South Korean wild boar. BMC Vet Res. 2015;11:78–82.
Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol. 2016;33:1870–4.
MacLachlan NJ, Dubovi EJ. Fenner’s veterinary virology. 4th ed. Amsterdam: Elsevier Inc.; 2011. p. 353.
Mengeling WL, Ridpath JF, Vorwald AC. Size and antigenic comparison among the structural proteins of selected autonomous parvoviruses. J Gen Virol. 1988;69:825–7.
Palinski RM, Mitra N, Hause BM. Discovery of a novel Parvovirinae virus porcine parvovirus 7 by metagenomics sequencing of porcine rectal swabs. Virus Genes. 2016;52:564–7.
Shangjin C, Cortey M, Segalés J. Phylogeny and evolution of the NS1 and VP1/VP2 gene sequences from porcine parvovirus. Virus Res. 2009;40:209–15.
Sharma R, Saikumar G. Parvovirus and PCV2 in crossbred Indian pigs. Trop Anim Health Prod. 2010;42:515–22.
Soares RM, Durigon EL, Bersano JG, Richtzenhain LJ. Detection of porcine parvovirus DNA by the polymerase chain reaction assay using primers to the highly conserved non-structural protein gene NS-1. J Virol Methods. 1999;78:191–8.
Xu XG, Chen GD, Huang Y, Ding L, Li ZC, Chang CD, Wang CY, Tong DW, Liu HJ. Development of multiplex PCR for simultaneous detection of six swine DNA and RNA viruses. J Virol Methods. 2012;183:69–74.
Acknowledgments
The authors thank the Dean, College of Veterinary and Animal Sciences, Pookode for providing the facilities for conduct of the study.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Aishwarya, J., Ravishankar, C., Rajasekhar, R. et al. First report of detection and molecular characterization of porcine parvovirus in domestic and wild pigs in Kerala, India. VirusDis. 27, 311–314 (2016). https://doi.org/10.1007/s13337-016-0330-z
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13337-016-0330-z