Skip to main content
SpringerLink
Log in

Heterozygosity at the SNP (rs136500299) of ITGB6 receptor gene possibly influences the susceptibility among crossbred bull to foot and mouth disease infection

  • Original Article
  • Published:
VirusDisease Aims and scope Submit manuscript

Abstract

Zebu (Bos indicus) cattle are known to be resistant against foot and mouth disease virus (FMDV) compared to taurine (Bos taurus). To understand the susceptibility of two cattle species to FMDV infection in terms of viral receptors, the present study reports the cloning, characterization and sequence analysis of Zebu ITGB6 gene. The complete CDS of zebu ITGB6 was 2367 basepair in length with 788 amino acid residues. The zebu integrin shares common structural and functional elements with taurine and other species. We identified an amino substitution (S665 to F665) presents in ITGB6 gene among zebu and taurine as SNP (rs136500299). Further, we determined and compared the structural differences of ITGB6 receptor gene among zebu and taurine species. To elucidate the influence of the SNP on the susceptibility of cattle to FMDV infection, a tetra ARMS PCR based genetic screening was performed among Zebu and crossbred cattle. Our observation revealed that, the targeted SNP are strongly (P < 0.05) associated with FMD susceptibility among Frieswal (HF X Sahiwal) crossbred cattle.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Berinstein A, Roivainen M, Hovi T, Mason PW, Baxt B. Antibodies to the vitronectin receptor (integrin avb3) inhibit binding and infection of foot-and mouth disease virus to cultured cells. J Virol. 1995;1995(69):2664–6.

    Google Scholar 

  2. Du J, Chang H, Gao S, Cong G, Shao J, Lin T, Liu Z, Liu X, Cai X. Sheep (Ovisaries) integrins alphavbeta1 and alphavbeta6 related to foot-and-mouth disease virus infection: molecular cloning, sequence analysis and comparison with homologues. Mol Cell Probes. 2009;23(5):247–57.

    Article  CAS  PubMed  Google Scholar 

  3. Du J, Gao S, Chang H, Cong G, Lin T, Shao J, Liu Z, Liu X, Cai X. Bactrian camel (Camelusbactrianus) integrins alphavbeta3 and alphavbeta6 as FMDV receptors: molecular cloning, sequence analysis and comparison with other species. Vet ImmunolImmunopathol. 2009;131(3–4):190–9.

    Article  CAS  Google Scholar 

  4. Du J, Larska M, Chang H, Alexandersen S, Cai X. Molecular cloning and phylogenetic analysis of integrins alphavbeta1 and alphavbeta6 of one-humped camel (Camelusdromedarius). Vet ImmunolImmunopathol. 2010;135(1–2):164–71.

    Article  CAS  Google Scholar 

  5. Duque H, Baxt B. Foot-and-mouth disease virus receptors: comparison of bovine av integrin utilization by type A and O viruses. J Virol. 2003;77:2500–11.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  6. Hynes RO. Integrins: versatility, modulation, and signaling in cell adhesion. Cell. 1992;69:11–25.

    Article  CAS  PubMed  Google Scholar 

  7. Jackson T, Clark S, Berryman S, Burman A, Cambier S, Mu D, et al. Integrin avb8 functions as a receptor for foot-and-mouth disease virus: role of the b-chain cytodomain in integrin-mediated infection. J Virol. 2004;78:4533–40.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  8. Jackson T, Mould AP, Sheppard D, King AMQ. Integrin avb1 is a receptor for foot-and-mouth disease virus. J Virol. 2002;2002(76):935–41.

    Article  Google Scholar 

  9. Jackson T, Sheppard D, Denyer M, BlakemoreW King AMQ. The epithelial integrin avb6 is a receptor for foot-and-Mouth disease virus. J Virol. 2000;74:4949–56.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  10. Longjam N, Deb R, Sarmah AK, Tayo T, Awachat VB, Saxena VK. A brief review on diagnosis of foot-and-mouth disease of livestock: conventional to molecular tools. Vet Med Int. 2011;2011(2011):905768. doi:10.4061/2011/905768.

    PubMed Central  PubMed  Google Scholar 

  11. Oshima M, Banno S, Okada K, Takeuchi T, Kimura M, Ichiishi A, Yamaguchi I, Fujimura M. Survey of mutations of a histidine kinase gene BcOS1 in dicarboximide-resistant field isolates of Botrytis cinerea. J Gen Plant Pathol. 2006;72:65–73.

    Article  CAS  Google Scholar 

  12. Saito S, Suzuki S, Takayanagi T. Nested PCR-RFLP is a high-speed method to detect fungicide-resistant Botrytis cinerea at an early growth stage of grapes. Pest Manag Sci. 2009;65:197–204.

    Article  CAS  PubMed  Google Scholar 

  13. Sambrook J, Russel DW. Molecular cloning: a laboratory manual. 3rd ed. New York: Cold Spring Harbor Laboratory Press; 2001.

    Google Scholar 

  14. Singh R, Deb R, Singh U, Alex R, Kumar S, Chakraborti S, Sharma S, Sengar G, Singh R. Development of tetra ARMS PCR based detection of a novel SNP at 5′UTR region of bovine ITGB6 receptor gene associated with Foot and Mouth Disease susceptibility in cattle. Archieves of Virology. 2014;159:3385–9. doi:10.1007/s00705-014-2194-0.

    Article  CAS  Google Scholar 

  15. Stewart PL, Nemerow GR. Cell integrins: commonly used receptors for diverse viral pathogens. Trends Microbiol. 2007;15(11):500–7.

    Article  CAS  PubMed  Google Scholar 

  16. Thomson GR, Vosloo W, Bastos ADS. Foot and mouth disease in wildlife. Virus Res. 2003;91:145–61.

    Article  CAS  PubMed  Google Scholar 

  17. Ye S, Dhillon S, Ke X, Collins AR, Day IN. An efficient procedure for genotyping single nucleotide polymorphisms. Nucleic Acids Res. 2001;29(17):E88.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

The project (SR/WOS-A/LS-437/2012 -G) was financially supported by Department of Science and Technology, Govt. of India. The authors are thankful to Director, CIRC, Meerut for providing necessary facilities for conducting the study.

Author information

Authors and Affiliations

  1. Molecular Genetics Laboratory, Central Institute for Research on Cattle, Indian Council of Agricultural Research, Meerut, 250 001, Uttar Pradesh, India

    Rani Singh, Rajib Deb, Umesh Singh, T. V. Raja, Rani Alex, Sushil Kumar, Rafeeque R. Alyethodi, Sheetal Sharma & Gyanendra Sengar

  2. Indian Veterinary Research Institute, Izatnagar, 243122, Uttar Pradesh, India

    Soumendu Chakraborti

Authors
  1. Rani Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Rajib Deb
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Umesh Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. V. Raja
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rani Alex
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Sushil Kumar
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Soumendu Chakraborti
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Rafeeque R. Alyethodi
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Sheetal Sharma
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Gyanendra Sengar
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Rajib Deb.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Singh, R., Deb, R., Singh, U. et al. Heterozygosity at the SNP (rs136500299) of ITGB6 receptor gene possibly influences the susceptibility among crossbred bull to foot and mouth disease infection. VirusDis. 26, 48–54 (2015). https://doi.org/10.1007/s13337-015-0249-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13337-015-0249-9

Keywords

Search

Navigation