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Hsp70 expression in shrimp Litopenaeus vannamei in response to IHHNV and WSSV infection

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Abstract

The effects of environmental changes, on non-target organisms may impact and bring consequences at the molecular level in marine organisms. There is a lack of data supporting the hypothesis according to which environmental stress modulates the immune system, leading to an increased susceptibility to infectious agents in shrimps. The present study was focused on changes occurring in cellular defense proteins in Litopenaeus vannamei infected by virus infection hypodermal and hematopoietic necrosis (IHHNV) and white spot syndrome (WSSV). Western blot analysis was used to evaluate expression of protein Hsp70, in gill tissue. Up-regulation levels were supported by immune detection analysis, suggesting that IHHNV and WSSV-shrimp infection promotes changes in the expression of these proteins.

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Acknowledgments

We would like to thank CNPq-Brazilian Council for the Scientific and Technological Development and FINEP—National Financing Fund for Studies and Projects for supporting this research (CNPq-Grant Proc.480416/2007-9; FINEP-Grant n.01070625-00/336-2007). PAVN was recipient of CAPES (Amazônia Azul) and JRM was recipient of CAPES fellowship. MRFM is recipient of CNPq productivity fellowship. The authors would like to thank Aristides Leandro da Silva Filho and Frank Belettini for assistance during the experiment set up and for shrimp specimens.

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Authors and Affiliations

  1. LABCAI, Dep. Bioquímica, CCB, NEPAq, Universidade Federal de Santa Catarina, Florianópolis, SC, 88034-257, Brazil

    Pedro A. Valentim-Neto, Juliana R. Moser, Ana P. M. Fraga & Maria R. F. Marques

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  1. Pedro A. Valentim-Neto
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  2. Juliana R. Moser
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  3. Ana P. M. Fraga
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  4. Maria R. F. Marques
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Correspondence to Pedro A. Valentim-Neto.

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Valentim-Neto, P.A., Moser, J.R., Fraga, A.P.M. et al. Hsp70 expression in shrimp Litopenaeus vannamei in response to IHHNV and WSSV infection. VirusDis. 25, 437–440 (2014). https://doi.org/10.1007/s13337-014-0236-6

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  • DOI: https://doi.org/10.1007/s13337-014-0236-6

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