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Detection and Molecular Characterization of Porcine Picobirnavirus in Feces of Domestic Pigs from Kolkata, India

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Abstract

Picobirnaviruses (PBVs) are small, non-enveloped, 35–41 nm virion with bisegmented double-stranded RNA genome. PBVs are widespread and were detected in feces of humans and a wide variety of animals. Domestic pig, one of the ubiquitous farm animal reported incessant association with a variety of viral zoonoses. The objective of our study is to find out the incidence of PBV infection in healthy domestic pigs. The study was conducted by collecting feces of healthy/asymptomatic pigs from a piggery located in an urban slum at Kolkata, India to detect PBV infections. All the 11 fecal samples were tested by polyacrylamide gel electrophoresis and reverse transcription–polymerase chain reaction assay. In this study, we report the first incidence of detection and molecular characterization of porcine PBV (BG-Por-2/2010 and BG-Por-7/2010) in feces of domestic pigs from India using the human PBV genogroup I specific primer pair: PicoB25(+) and PicoB43(−). Sequence comparison and phylogenetic analysis of partial RNA-dependent RNA polymerase gene of genome segment 2 revealed genetic relatedness to hitherto reported porcine, murine and human genogroup I PBVs from different geographical regions. This warrants a stringent global surveillance to study the potential zoonotic and emerging PBV infections.

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Acknowledgments

We are grateful to Tapan Dutta, Debshis Khatua, Md. Azim and Dipak Das who helped in organizing, collection and transport of porcine fecal specimens to the Virology laboratory for processing. We sincerely acknowledge the technical assistance of Bimal Bera, Khokon Sen, Mussaraf Hossain, Neh Nupur, Chandrima Ghosh, Durga Poddor, Srinwanthi Bhattacharya, Arpan Kahali and the staff members of Division of Virology for their whole-hearted support. The study was financially supported by Indian Council of Medical Research (ICMR, Govt. of India), Japan International Co-operation Agency (JICA, Govt. of Japan) and Program of Founding Research Centre for Emerging and Reemerging Infectious Disease (Okayama University—NICED, India) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.

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Authors and Affiliations

  1. Division of Virology, National Institute of Cholera and Enteric Diseases (NICED), P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata, 700 010, West Bengal, India

    Balasubramanian Ganesh

  2. Veterinary Medical Research Institute, Hungarian Academy of Sciences, Hungaria krt. 21, Budapest, 1143, Hungary

    Krisztián Bányai

  3. Division of Epidemiology, National Institute of Cholera and Enteric Diseases (NICED), P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata, 700 010, West Bengal, India

    Suman Kanungo & Deepika Sur

  4. Division of Virology, Indian Veterinary Research Institute (IVRI), Mukteswar, Nainital, Uttarakhand, 263 138, India

    Yashpal Singh Malik

  5. Department of Hygiene, Sapporo Medical University School of Medicine, S-1 W-17, Chuo-Ku, Sapporo, 060-8556, Japan

    Nobumichi Kobayashi

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  1. Balasubramanian Ganesh
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  2. Krisztián Bányai
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  3. Suman Kanungo
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  4. Deepika Sur
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  5. Yashpal Singh Malik
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  6. Nobumichi Kobayashi
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Correspondence to Balasubramanian Ganesh.

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Ganesh, B., Bányai, K., Kanungo, S. et al. Detection and Molecular Characterization of Porcine Picobirnavirus in Feces of Domestic Pigs from Kolkata, India. Indian J. Virol. 23, 387–391 (2012). https://doi.org/10.1007/s13337-012-0106-z

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  • DOI: https://doi.org/10.1007/s13337-012-0106-z

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