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Functional Characterization of Interferon Regulation Element of Hepatitis B virus Genome In Vivo

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Abstract

The roles of interferon regulatory element (IRE) in Hepatitis B virus (HBV) genome on inhibitory effect of interferon against HBV are controversial in vitro. This study aimed to determine the functional characterization of HBV-IRE sequence in vivo. Wild-type or IRE-mutant HBV replication-competent mice were firstly established, and mice were subquently treated with polyinosinic-polytidylin acid (polyI.C) or phosphate-buffered saline via intraperitoneal. Results showed that PolyI.C inhibited viral replication, and increased the level of 2′,5′-oligoadenylate synthase mRNA transcripts, a marker of INF-α/β induction. Between wild-type and IRE-mutant HBV replication-competent mice, the levels of HBV-RNA and HBV-DNA replication intermediates were similar. After PolyI.C treatment, the decreasing of HBV-RNA was similar between two groups, but HBV-DNA replication intermediates decreased significantly less in IRE-mutant than wild-type HBV replication-competent mice. These findings suggested that IRE mutant reduced the inhibitory effect of interferon on HBV replication, which played a role in antiviral effect of interferon against HBV.

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Acknowledgments

This work was supported by National Key Technologies Research and Development Program of China (2012ZX10002007 and 2008ZX10002-006), National Natural Science Foundation of China (No. 30972622), and National Basic Research Program of China (No.2007CB512902).

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Authors and Affiliations

  1. Center of Infectious Diseases, West China Hospital of Sichuan University, No.37 Guo Xue Xiang, Chengdu, 610041, People’s Republic of China

    Feng-Jun Liu, En-Qiang Chen, Qiao-Ling Zhou, Tao-You Zhou, Cong Liu, Li Liu, Xing Cheng & Hong Tang

  2. Division of Infectious Diseases, State Key Laboratory of Biotherapy, Sichuan University, Chengdu, 610041, People’s Republic of China

    Feng-Jun Liu, En-Qiang Chen, Qiao-Ling Zhou, Tao-You Zhou, Cong Liu, Li Liu, Xing Cheng & Hong Tang

  3. Department of Infectious Diseases, North Sichuan Medical College, Nanchong, 637000, People’s Republic of China

    Feng-Jun Liu

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  1. Feng-Jun Liu
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Correspondence to Hong Tang.

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Feng-Jun Liu, En-Qiang Chen contributed equally to this paper.

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Liu, FJ., Chen, EQ., Zhou, QL. et al. Functional Characterization of Interferon Regulation Element of Hepatitis B virus Genome In Vivo. Indian J. Virol. 23, 278–285 (2012). https://doi.org/10.1007/s13337-012-0091-2

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  • DOI: https://doi.org/10.1007/s13337-012-0091-2

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