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New Experimental Hosts of Tobacco streak virus and Absence of True Seed Transmission in Leguminous Hosts

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Abstract

Of 70 plant species tested, 50 species were susceptible to Tobacco streak virus (TSV) on sap inoculation. Both localized (necrotic and chlorotic spots) and systemic (necrotic spots, axillary shoot proliferation, stunting, total necrosis and wilt) symptoms are observed by majority of plant species. Eleven new experimental hosts were identified viz., Amaranthus blitum var. oleracea (Chaulai sag), Celosia cristata (Cocks comb), Beta vulgaris var. bengalensis (Palak/Indian spinach), Calendula officinalis (Pot marigold), Chrysanthemum indicum, Cosmos sulphurens (Yellow cosmos), Citrullus lunatus (Watermelon), Lagenaria siceraria (Bottle gourd), Coriandrum sativum (Coriander), Hibiscus subderiffa var. subderiffa (Roselle) and Portulaca oleraceae (Little hogweed). Detected groundnut seed infection with TSV for the first time by Direct antigen coated immunosorbent assay (DAC-ELISA) using whole seed. The seed infection ranged from 18.9 to 28.9% among the seeds collected from naturally infected and sap inoculated groundnut varieties (JL 24, TMV 2, Prasuna, Kadiri 6, Kadiri 9, Anantha and Kadiri 7 Bold) belonging to spanish and virginia types. Further, TSV was detected both in pod shell and seed testa and none of the samples showed the presence of TSV either in cotyledon or embryo. Grow-out and bio-assay tests proved the absence of seed transmission in groundnut and other legume crops. Hence, TSV isolate was not a true seed transmission case under Indian conditions in legumes.

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Acknowledgments

Greatly indebted to the Indian Agricultural Research Institute, New Delhi and Acharya N.G. Ranga Agricultural University, Andhra Pradesh for financial support and to Dr. R.D.V.J Prasada Rao, Principal Scientist and Dr. M. Johnson, Senior Scientist for their generous help. I thank to Dr. K. Sankar Reddy, Associate Director of Research, and Dr. D. Lokanadha Reddy, Principal Scientist (Groundnut) and Head for critical reading of the manuscript and encouragement.

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Authors and Affiliations

  1. Acharya N.G. Ranga Agricultural University, Agricultural Research Station, Kadiri, 515591, Andhra Pradesh, India

    K. Vemana

  2. Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India

    R. K. Jain

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  1. K. Vemana
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  2. R. K. Jain
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Correspondence to R. K. Jain.

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Vemana, K., Jain, R.K. New Experimental Hosts of Tobacco streak virus and Absence of True Seed Transmission in Leguminous Hosts. Indian J. Virol. 21, 117–127 (2010). https://doi.org/10.1007/s13337-010-0021-0

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  • DOI: https://doi.org/10.1007/s13337-010-0021-0

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