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Detection of Rotavirus Infection in Bovine Calves by RNA-PAGE and RT-PCR

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Abstract

A total of 128 diarrhoeic faecal samples were collected from cattle and buffalo calves from Pantnagar and Dehradun during winter months. Of the 110 cattle calves screened by RNA-PAGE, rotavirus was detected in 13 samples (11.81%) while no sample from buffalo calves was found positive. All samples were found to have long electropherotype and two distinct electropherotypes having segment variation were observed. The overall prevalence of rotavirus was 10.15% (13/128). RT-PCR targeting group specific VP6 gene confirmed Group A rotavirus in 10 out of 13 samples, while three samples remained un-groupable.

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Authors and Affiliations

  1. Department of Veterinary Microbiology, College of Veterinary and Animal Science, G. B. Pant University of Agriculture and Technology, Pantnagar, U. S. Nagar, Uttarakhand, India

    Sanjay Singh Basera & Rashmi Singh

  2. Division of Virology, Indian Veterinary Research Institute, Mukteshwar, Nainital, 263138, India

    N. Vaid, K. Sharma, S. Chakravarti & Y. P. S. Malik

Authors
  1. Sanjay Singh Basera
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  2. Rashmi Singh
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  3. N. Vaid
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  4. K. Sharma
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  5. S. Chakravarti
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  6. Y. P. S. Malik
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Correspondence to Rashmi Singh.

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Basera, S.S., Singh, R., Vaid, N. et al. Detection of Rotavirus Infection in Bovine Calves by RNA-PAGE and RT-PCR. Indian J. Virol. 21, 144–147 (2010). https://doi.org/10.1007/s13337-010-0017-9

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  • DOI: https://doi.org/10.1007/s13337-010-0017-9

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