Abstract
The method of extracting nematodes from the soil developed by Jenkins was combined with the Coolen and D’Herde technique with the aim of optimising the extraction of heteroderid cysts from the soil. In addition to the soil, the combined methodology employs 40-mesh (420 μm) and 200-mesh (74 μm) sieves, kaolin and sucrose. Using this method, it is possible to obtain quickly almost four times the number of cysts in an almost clear suspension, compared to the traditional method of cyst-extraction devised by Shepherd.
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23 November 2022
A Correction to this paper has been published: https://doi.org/10.1007/s13313-022-00896-3
References
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Universidade Federal do Ceará (UFC), Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
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da Silva Café, F.B., Santos, C.D.G. Method for extracting nematode cysts of the family Heteroderidae from sandy loam soil. Australasian Plant Pathol. 52, 43–44 (2023). https://doi.org/10.1007/s13313-022-00861-0
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DOI: https://doi.org/10.1007/s13313-022-00861-0