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Survival, transmission and control of Phoma koolunga in field pea seed and reaction of field pea genotypes to the pathogen

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Abstract

Little is known about the epidemiology of Phoma koolunga, a component of the ascochyta blight complex of field pea in southern Australia. The aims of this research were to investigate seed infection, efficacy of fungicides as seed dressings and the reaction of current field pea genotypes to this fungus. The frequency of isolation of P. koolunga from individual seed samples from South Australia, Victoria and Western Australia ranged from 0 to 6 %. Disease was transmitted to 98 % of seedlings that emerged from artificially inoculated seeds (AIS) in growth room conditions. The necrotic index on seedlings that emerged from AIS at 8 °C was greater than that for seedlings at 12, 16 and 20 °C. P-Pickel T® and Jockey Stayer® were the most effective fungicides for reducing disease incidence and severity on seedlings emerged from AIS sown in soil and on germination paper, respectively. The response of 12 field pea genotypes to one isolate of P. koolunga was assessed by spraying plants with pycnidiospore suspension in controlled conditions and examining symptoms from 3 to 21 days post-inoculation (dpi). Genotypes Sturt, Morgan and Parafield showed more severe disease on the lowest three leaves than the other genotypes at 21 dpi. In another experiment, four genotypes of short, semi-leafless type field peas were inoculated with three isolates of P. koolunga which differed in virulence and assessed as described above. Kaspa showed significantly less disease than Morgan or WAPEA2211 at 21 dpi when inoculated with two of the three isolates tested. Isolates of P. koolunga differed in aggressiveness based on % leaf area diseased until 14 dpi, but differences were not significant at 21 dpi.

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References

  • Adhikari KN, Khan TN, Stefanova K, Pritchard I (2014) Recurrent breeding method enhances the level of blackspot (Didymella pinodes (berk. & blox.) vestergr.) resistance in field pea (Pisum sativum L.) in southern Australia. Plant Breed 133:508–514

    Article  Google Scholar 

  • Ali SM, Dennis J (1992) Host range and physiological specialization of Macrophomina phaseolina isolated from field peas in South Australia. Australi J Exp Agric 32:1121–1125

    Article  Google Scholar 

  • Ali SM, Paterson J, Crosby J (1982) A standard technique for detecting seed-borne pathogens in peas, chemical control, and testing commercial seed in South Australia. Australi J Exp Agric 22:348–352

    Article  Google Scholar 

  • Bretag TW, Keane PJ, Price TV (2006) The epidemiology and control of ascochyta blight in field peas: a review. Aust J Agric Res 57:883–902

    Article  Google Scholar 

  • Bretag TW, Price TV, Keane PJ (1995) Importance of seed-borne inoculum in the etiology of the ascochyta blight complex of field peas (Pisum sativum L) grown in Victoria. Australi J Exp Agric 35:525–530

    Article  Google Scholar 

  • Davidson J, Kimber RE (2007) Integrated disease management of ascochyta blight in pulse crops. Eur J Plant Pathol 119:99–110

    Article  Google Scholar 

  • Davidson JA, Hartley D, Priest M, Herdina K-KM, Mckay A, Scott ES (2009) A new species of Phoma causes ascochyta blight symptoms on field peas (Pisum sativum) in South Australia. Mycologia 101:120–128

    Article  CAS  PubMed  Google Scholar 

  • Davidson JA, Krysinska-Kaczmarek M, Wilmshurst CJ, Mckay A, Herdina SES (2011) Distribution and survival of ascochyta blight pathogens in field pea cropping soils of Australia. Plant Dis 95:1217–1223

    Article  Google Scholar 

  • Davidson JA, Wilmshurst CJ, Scott ES, Salam MU (2013) Relationship between ascochyta blight on field pea (Pisum sativum) and spore release patterns of Didymella pinodes and other causal agents of ascochyta blight. Plant Pathol 62:1258–1270

    Article  Google Scholar 

  • Fry W (1978) Quantification of general resistance of potato cultivars and fungicide effects for integrated control of potato late blight. Phytopathol 68:1650–1655

    Article  CAS  Google Scholar 

  • Gossen BD, Mcdonald MR, Conner RL, Hwang S-F, Chang K-F (2010) Significance of seed infection on epidemics of mycosphaerella blight in field pea. Can J Plant Pathol 32:458–467

    Article  Google Scholar 

  • Gossen BD, Morrall RA (1986) Transmission of Ascochyta lentis from infected lentil seed and plant residue. Can J Plant Pathol 8:28–32

    Article  Google Scholar 

  • Hawthorne W, Davidson J, Lindbeck K (2010) Pulse Seed treatments & Foliar Fungicides http://www.sardi.sa.gov.au/__data/assets/pdf_file/0005/74921/Pulse_Seed_treatments__and__Foliar_Fungicides_-_7th_Edition.pdf

  • Hawthorne W, Davidson J, Mcmurray L, Armstrong E, Macleod B, Richardson HJ (2012) Field pea disease management strategy, Southern and Western region http://www.pulseaus.com.au/pdf/Field%20pea%20Disease%20Management%20Strategy%20Southern%20&%20Western%20Region.pdf

  • Hwang SF, Lopetinsky K, Evans IR (1991) Effects of seed infection by Ascochyta spp., fungicide seed treatment, and cultivar on yield parameters of field pea under field conditions. Can Plant Dis Surv 71:169–172

    Google Scholar 

  • Kaiser WJ (1997) Inter- and intranational spread of ascochyta pathogens of chickpea, faba bean, and lentil. Can J Plant Pathol 19:215–224

    Article  Google Scholar 

  • Kimber RBE, Ramsey MD (2001) Using fungicides to control ascochyta blight of chickpea. Proceedings of the 13th Australasian Plant Pathology Conference. Cairns, QLD, Australia, 199

  • Kimber RBE, Scott ES, Ramsey MD (2006) Factors influencing transmission of Didymella rabiei (ascochyta blight) from inoculated seed of chickpea under controlled conditions. Eur J Plant Pathol 114:175–184

    Article  Google Scholar 

  • Kimber RBE, Shtienberg D, Ramsey MD, Scott ES (2007) The role of seedling infection in epiphytotics of ascochyta blight on chickpea. Eur J Plant Pathol 117:141–152

    Article  Google Scholar 

  • Knappe B, Hoppe HH (1995) Investigations on the resistance of peas (P. sativum L.) toward Ascochyta pinodes and Phoma medicaginis var. pinodella. In. Proceedings of the 2nd European Conference on Grain Legumes. Copenhagen, Denmark: AEP, 86–87.

  • Knott CM (1987) A key for stages of development of the pea (Pisum sativum). Ann Appl Biol 111:233–245

    Article  Google Scholar 

  • Moussart A, Tivoli B, Lemarchand E, Deneufbourg F, Roi S, Sicard G (1998) Role of seed infection by the ascochyta blight pathogen of dried pea (Mycosphaerella pinodes) in seedling emergence, early disease development and transmission of the disease to aerial plant parts. Eur J Plant Pathol 104:93–102

    Article  Google Scholar 

  • Onfroy C, Tivoli B, Corbière R, Bouznad Z (1999) Cultural, molecular and pathogenic variability of Mycosphaerella pinodes and Phoma medicaginis var. pinodella isolates from dried pea (Pisum sativum) in France. Plant Pathol 48:218–229

    Article  Google Scholar 

  • Ophel-Keller K, Mckay A, Hartley D, Herdina CJ (2008) Development of a routine DNA-based testing service for soilborne diseases in Australia. Australas Plant Pathol 37:243–253

    Article  CAS  Google Scholar 

  • Priestley RH, Bayles RA, Sweet JB, Knight C, Parry DW, Wright IR, Tierney PI, Kerr SP, Gans PT, Wooster P (1985) Disease assessment manual for crop variety trials. National lnstitute of Agricultural Botany. United Kingdom, Cambridge

    Google Scholar 

  • Richardson HJ, Leonforte T, Smith AJ (2009) Response of field pea varieties to the fungal components of the ascochyta complex In. The Second International Ascochyta Workshop. Washington, USA: Washington State University, 60.

  • Salam MU, Macleod WJ, Maling T, Prichard I, Seymour M, Barbetti MJ (2011a) A meta-analysis of severity and yield loss from ascochyta blight on field pea in Western Australia. Australas Plant Pathol 40:591–600

    Article  Google Scholar 

  • Salam MU, Macleod WJ, Pritchard I, Seymour M, Davidson JA, Salam KP, Galloway J, Mcmurray LS, Lindbeck KD, Richardson H (2011b) G2 blackspot manager model to guide field pea sowing for southern Australia in relation to ascochyta blight disease. Australas Plant Pathol 40:632–639

    Article  Google Scholar 

  • Schoeny A, Jumel S, Rouault F, Le May C, Tivoli B (2007) Assessment of airborne primary inoculum availability and modelling of disease onset of ascochyta blight in field peas. Eur J Plant Pathol 119:87–97

    Article  Google Scholar 

  • Shaner G, Finney R (1977) The effect of nitrogen fertilization on the expression of slow-mildewing resistance in knox wheat. Phytopathol 67:1051–1056

    Article  CAS  Google Scholar 

  • Sprague SJ, Burgess DR (2001) Seed treatment to suppress infection of canola seedlings by Leptosphaeria maculans. In: Marcroft SJ, ed. 12th Australian research assembly on brassicas. Geelong, Victoria, Australia, 68–72.

  • Tivoli B, Banniza S (2007) Comparison of the epidemiology of ascochyta blights on grain legumes. Eur J Plant Pathol 119:59–76

    Article  Google Scholar 

  • Tivoli B, Baranger A, Avila CM, Banniza S, Barbetti M, Chen WD, Davidson J, Lindeck K, Kharrat M, Rubiales D, Sadiki M, Sillero JC, Sweetingham M, Muehlbauer FJ (2006) Screening techniques and sources of resistance to foliar diseases caused by major necrotrophic fungi in grain legumes. Euphytica 147:223–253

    Article  Google Scholar 

  • Tran HS, Li YP, You MP, Khan TN, Pritchard I, Barbetti MJ (2014) Temporal and spatial changes in the pea black spot disease complex in Western Australia. Plant Dis 98:790–796

    Article  Google Scholar 

  • Wallen VR, Cuddy TF, Grainger PN (1967) Epidemiology and control of Ascochyta pinodes on field peas in Canada. Can J Plant Sci 47:395–403

    Article  Google Scholar 

  • Xue AG (2000) Effect of seed-borne Mycosphaerella pinodes and seed treatments on emergence, foot rot severity, and yield of field pea. Can J Plant Pathol 22:248–253

    Article  Google Scholar 

Download references

Acknowledgments

This research was a part of the PhD project of the first author, supported by a scholarship from Iranian Ministry of Science, Research and Technology. The authors thank Dr. R. Kimber and Mrs. M. Krysinska-Kaczmarek for technical advice, and Dr. Herdina for DNA testing of samples.

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Authors and Affiliations

  1. School of Agriculture, Food and Wine, Waite Research Institute, The University of Adelaide, South Australia, 5005, Australia

    M. Khani, J. A. Davidson, M. R. Sosnowski & E. S. Scott

  2. South Australian Research and Development Institute, GPO Box 397, Adelaide, South Australia, 5001, Australia

    J. A. Davidson & M. R. Sosnowski

  3. Darab College of Agriculture and Natural Resources, Shiraz University, Shiraz, Iran

    M. Khani

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  1. M. Khani
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  2. J. A. Davidson
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  3. M. R. Sosnowski
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  4. E. S. Scott
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Correspondence to E. S. Scott.

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Khani, M., Davidson, J.A., Sosnowski, M.R. et al. Survival, transmission and control of Phoma koolunga in field pea seed and reaction of field pea genotypes to the pathogen. Australasian Plant Pathol. 45, 91–102 (2016). https://doi.org/10.1007/s13313-015-0394-z

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  • DOI: https://doi.org/10.1007/s13313-015-0394-z

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