Abstract
Anthracnose of grapevine, caused by the fungus Sphaceloma ampelinum de Bary, the anamorph stage of Elsinoe ampelina, is one of the major diseases of grapevine (Vitis vinifera L.) in the tropics. Ten grapevine cultivars/lines and 24 F1 hybrids were evaluated for resistance to anthracnose in the laboratory, using an excised leaf technique with two anthracnose isolates (Nk4-1 and Rc2-1), and in the field by using natural infection during 2007 and 2009. Significant differences in the lesion/disease scores among grapevine genotypes, ranging from 1 (resistant) to 5 (susceptible), were observed under both laboratory and field conditions, but ranking of genotypes may vary between screening methods. No significant difference in disease severity was observed among three consecutive years of field evaluations. Resistance evaluations under both conditions consistently classified ‘Wilcox 321’, ‘NY88.0507.01’, ‘NY65.0550.04’ and ‘Illinois 547-1’ as resistant lines useful as parents for future breeding programs. Moreover, one F1 hybrid ‘SUT0404.40’, was found to be resistant to both isolates under laboratory and field evaluations. The resistance levels of 34 grape genotypes evaluated under laboratory (using isolates Nk4-1 and Rc2-1) and field conditions gave consistent results with Spearman’s rank correlation coefficients of 0.72 and 0.71 (P ≤ 0.01), respectively, suggesting that this laboratory screening assay is efficient for rapid, reliable and economical identification of resistant hybrids in grapevine breeding programs.
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Acknowledgements
We are very grateful to Dr. Bruce I. Reisch for providing resistant line cuttings and advice. This research is partially supported by the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, the Center of Excellence on Agricultural Biotechnology, Science and Technology Postgraduate Education and Research Development Office, Office of the Higher Education Commission, Ministry of Education (AG-BIO/PERDO-CHE), and grants from Suranaree University of Technology, Thailand.
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Poolsawat, O., Tharapreuksapong, A., Wongkaew, S. et al. Laboratory and field evaluations of resistance to Sphaceloma ampelinum causing anthracnose in grapevine. Australasian Plant Pathol. 41, 263–269 (2012). https://doi.org/10.1007/s13313-012-0127-5
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DOI: https://doi.org/10.1007/s13313-012-0127-5