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Overexpression and biological function of TMEM48 in non-small cell lung carcinoma

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Tumor Biology

Abstract

Transmembrane protein 48 (TMEM48), localized to nuclear pore complexes (NPCs), has been reported crucial for NPC assembly. Alterations in NPC members have been reported in several malignancies. The present study was aimed to elucidate the expression and biological function of TMEM48 in non-small cell lung carcinoma (NSCLC). Here, TMEM48 expression level was higher in NSCLC tissues than that in the adjacent normal tissues. Moreover, higher TMEM48 expression was correlated with a more advanced tumor stage, lymph node metastasis, bigger tumor size tumor stage, and shorter survival time. Knockdown of TMEM48 in NSCLC cell lines, A549 and H1299, inhibited cell proliferation and significantly increased cells population in G1 phase. Gene set enrichment analysis (GSEA) showed that cell cycle pathway was correlative with the TMEM48 expression. Additionally, real-time PCR and western blot analysis revealed that several cell cycle and DNA replication genes, including Cyclin B1, CDK1, CDC6, PCNA, and RCF4, were reduced after TMEM48 knockdown. Additionally, inhibition of TMEM48 in NSCLC cells significantly stimulated cell apoptosis, while notably repressed cell adhesion, migration, invasion, and tumorigenicity in nude mice. Our data provide insight into the biological relevance of TMEM48 in NSCLC progression and highlight its usefulness as a prognostic factor and potential therapeutic target in NSCLC.

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Acknowledgments

This work was supported by grants from National Natural Science Foundation of China (81372521). We would like to also thank JRDUN Biotech. (Shanghai, China) for their technical assistance on bioinformatics analysis.

Author contributions

Conceived and designed the experiments: LWX QL. Performed the experiments: WLQ YDH BWS WJ MT PC. Analyzed the data: WLQ JM HYH. Contributed reagents/materials/analysis tools: BBX WZZ. Wrote the paper: WLQ QL.

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None

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Authors and Affiliations

  1. Department of Thoracic Surgery, Shanghai First People’s Hospital, School of Medicine, Shanghai Jiao Tong University, 100 Haining Rd, Shanghai, 200080, China

    Wenliang Qiao, Yudong Han, Pei Chen, Haiyang Hu, Binbin Xu, Wenzhuo Zhu & Qiang Lin

  2. Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

    Wei Jin

  3. State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China

    Mi Tian

  4. Department of Cardiothoracic Surgery, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai, China

    Jie Min

  5. Department of Pulmonary Medicine, Shanghai Chest Hospital, Shanghai Jiao Tong University, 241 West Huaihai Rd, Shanghai, 200030, China

    Liwen Xiong

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  1. Wenliang Qiao
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  2. Yudong Han
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  3. Wei Jin
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  4. Mi Tian
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  7. Haiyang Hu
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  8. Binbin Xu
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  9. Wenzhuo Zhu
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  10. Liwen Xiong
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  11. Qiang Lin
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Corresponding authors

Correspondence to Liwen Xiong or Qiang Lin.

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Qiao, W., Han, Y., Jin, W. et al. Overexpression and biological function of TMEM48 in non-small cell lung carcinoma. Tumor Biol. 37, 2575–2586 (2016). https://doi.org/10.1007/s13277-015-4014-x

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  • DOI: https://doi.org/10.1007/s13277-015-4014-x

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