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MicroRNA-148a inhibits migration of breast cancer cells by targeting MMP-13

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Tumor Biology

Abstract

Breast cancer is a threat to the health of women, and metastasis of breast cancer cells plays an important role in the deterioration of breast cancer. MicroRNAs play a critical role in the tumorigenesis and development of breast cancer. MicroRNA-148a (miR-148a) is associated with the growth and metastasis of tumor cells. In the present study, we investigated the role of miR-148a in migration of breast cancer cells as well as the underlying mechanism. MiR-148a was found to inhibit the proliferation and migration of breast cancer cells. To further explore the mechanism through which miR-148a plays its antitumor role, matrix metalloproteinase-13 (MMP-13) was identified as a target of miR-148a by western blot and luciferase reporter assay. Moreover, silence of MMP-13 mimicked the effect of miR-148a, whereas overexpression of MMP-13 rescued the impaired migration caused by miR-148a. Our study demonstrates that miR-148a inhibits the migration of breast cancer cells by targeting MMP-13 and also lays theoretical foundation for further exploration for the function of miR-148a.

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Authors and Affiliations

  1. The Seventh Department of General Surgery, Shengjing Hospital of China Medical University, 36 Sanhao Street, Shenyang, 110004, People’s Republic of China

    Jinqi Xue, Xi Gu, Yang Zhang & Wenhai Zhang

  2. Department of Spine and Joint Surgery, Shengjing Hospital of China Medical University, Shenyang, 110004, People’s Republic of China

    Zhiguang Chen

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  1. Jinqi Xue
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  2. Zhiguang Chen
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  3. Xi Gu
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  4. Yang Zhang
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  5. Wenhai Zhang
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Correspondence to Wenhai Zhang.

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Xue, J., Chen, Z., Gu, X. et al. MicroRNA-148a inhibits migration of breast cancer cells by targeting MMP-13. Tumor Biol. 37, 1581–1590 (2016). https://doi.org/10.1007/s13277-015-3926-9

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  • DOI: https://doi.org/10.1007/s13277-015-3926-9

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