Skip to main content

Advertisement

SpringerLink
Log in

MiR-153 regulates metastases of gastric cancer through Snail

  • Original Article
  • Published:
Tumor Biology

Abstract

MicroRNAs (miRNAs) play critical roles in tumorigenesis and cancer metastases of gastric carcinoma (GC). Recently, miR-153 was reported as a tumor suppressor miRNA silenced in GC, whereas the underlying mechanisms are much lacking. Here, we reported that miR-153 levels were significantly decreased and Snail levels were significantly increased in GC specimens, compared to paired adjacent non-tumor gastric tissue. Moreover, the levels of miR-153 and Snail were inversely correlated. Bioinformatics analyses and luciferase reporter assay showed that miR-153 targeted the 3′-untranslated region (3′-UTR) of Snail messenger RNA (mRNA) to inhibit its translation. Overexpression of miR-153 inhibited Snail-mediated cell invasiveness, while depletion of miR-153 increased Snail-mediated cell invasiveness in both Transwell cell migration assay and scratch wound healing assay. Thus, our data suggest that miR-153 suppression in GC promotes Snail-mediated cancer metastases. Re-establishment of miR-153 levels in GC may suppress cancer invasion.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Zhao X, Li X, Yuan H. MicroRNAs in gastric cancer invasion and metastasis. Front Biosci. 2013;18:803–10.

    Article  CAS  Google Scholar 

  2. Liu G, Jiang C, Li D, Wang R, Wang W. MiRNA-34a inhibits EGFR-signaling-dependent MMP7 activation in gastric cancer. Tumour Biol. 2014;35:9801–6.

    Article  CAS  PubMed  Google Scholar 

  3. Mao D, Zhang Y, Lu H, Zhang H. Molecular basis underlying inhibition of metastasis of gastric cancer by anti-VEGFa treatment. Tumour Biol. 2014;35:8217–23.

    Article  CAS  PubMed  Google Scholar 

  4. Ye Y, Zhou X, Li X, Tang Y, Sun Y, Fang J. Inhibition of epidermal growth factor receptor signaling prohibits metastasis of gastric cancer via downregulation of MMP7 and MMP13. Tumour Biol. 2014;35:10891–6.

    Article  CAS  PubMed  Google Scholar 

  5. Wu W, Ding H, Cao J, Zhang W. FBXL5 inhibits metastasis of gastric cancer through suppressing Snail1. Cell Physiol Biochem. 2015;35:1764–72.

    Article  PubMed  Google Scholar 

  6. Xu XY, Zhang LJ, Yu YQ, et al. Down-regulated MAC30 expression inhibits proliferation and mobility of human gastric cancer cells. Cell Physiol Biochem. 2014;33:1359–68.

    Article  CAS  PubMed  Google Scholar 

  7. Zhou X, Xia Y, Su J, Zhang G. Down-regulation of miR-141 induced by helicobacter pylori promotes the invasion of gastric cancer by targeting STAT4. Cell Physiol Biochem. 2014;33:1003–12.

    Article  CAS  PubMed  Google Scholar 

  8. Cui Y, Chen J, He Z, Xiao Y. SUZ12 depletion suppresses the proliferation of gastric cancer cells. Cell Physiol Biochem. 2013;31:778–84.

    Article  CAS  PubMed  Google Scholar 

  9. Di Leva G, Croce CM. MiRNA profiling of cancer. Curr Opin Genet Dev. 2013;23:3–11.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Pereira DM, Rodrigues PM, Borralho PM, Rodrigues CM. Delivering the promise of miRNA cancer therapeutics. Drug Discov Today. 2013;18:282–9.

    Article  CAS  PubMed  Google Scholar 

  11. Mei Q, Li F, Quan H, Liu Y, Xu H. Busulfan inhibits growth of human osteosarcoma through miR-200 family microRNAs in vitro and in vivo. Cancer Sci. 2014;105:755–62.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Wang F, Xiao W, Sun J, Han D, Zhu Y. MiRNA-181c inhibits EGFR-signaling-dependent MMP9 activation via suppressing Akt phosphorylation in glioblastoma. Tumour Biol. 2014;35:8653–8.

    Article  CAS  PubMed  Google Scholar 

  13. Zhang Z, Sun J, Bai Z, et al. MicroRNA-153 acts as a prognostic marker in gastric cancer and its role in cell migration and invasion. OncoTargets and therapy. 2015;8:357–64.

    CAS  PubMed  PubMed Central  Google Scholar 

  14. Sinagra T, Merlo S, Spampinato SF, Pasquale RD, Sortino MA. High mobility group box 1 contributes to wound healing induced by inhibition of dipeptidylpeptidase 4 in cultured keratinocytes. Front Pharmacol. 2015;6:126.

  15. Zhang J, Zhang XH, Wang CX, Liu B, Fan XS, Wen JJ, Shi QL, Zhou XJ. Dysregulation of microRNA biosynthesis enzyme Dicer plays an important role in gastric cancer progression. Int J Clin Exp Pathol. 2014;7:1702–1707.

Download references

Conflicts of interest

None

Compliance with ethical standards

Patient’s consents and approval from the Institutional Research Ethics Committee were obtained.

Author information

Authors and Affiliations

  1. Department of General Surgery, the First Affiliated Hospital of Liaoning Medical University, Jinzhou, 121001, China

    Zhongli Wang

  2. Department of Developmental Biology, Liaoning Medical University, 40, Section 3, Songpo Road, Jinzhou, 121001, China

    Chao Liu

Authors
  1. Zhongli Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chao Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chao Liu.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Wang, Z., Liu, C. MiR-153 regulates metastases of gastric cancer through Snail. Tumor Biol. 37, 15509–15515 (2016). https://doi.org/10.1007/s13277-015-3846-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13277-015-3846-8

Keywords

Search

Navigation