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RETRACTED ARTICLE: Downregulation of microRNA-217 and microRNA-646 acts as potential predictor biomarkers in progression, metastasis, and unfavorable prognosis of human osteosarcoma

  • Original Article
  • Published:
Tumor Biology

This article was retracted on 05 November 2016

Abstract

Despite the progress in therapeutic targets, it remains dissatisfactory for most osteosarcoma patients with metastasis or recurrence osteosarcoma. Therefore, it is required to determine the involved mechanisms of osteosarcoma. The aim of this study was to investigate the expression level of MiR-217 and miR-646 and also their association with clinicopathological features in patients with osteosarcoma. Total RNA was purified from patients with osteosarcoma and noncancerous bone tissues, and then quantitative real-time PCR was applied to evaluate the expression level of microRNAs. Our result suggested that miR-217 expression was remarkably deceased in osteosarcoma bone tissue when compared with noncancerous bone tissues (mean ± SD 5.32 ± 1.231, 2.01 ± 0.78; P = 0.024) and miR-646 expression decreased in osteosarcoma bone tissue in comparison with normal tissues (mean ± SD 4.56 ± 1.45, 1.76 ± 1.24; P = 0.041). Our findings indicated that decreased expression of MiR-217 and miR-646 was strongly correlated with high tumor, node, and metastasis (TNM) stage (P = 0.015, P = 0.002) and large cancer diameter (P = 0.041, P = 0.053). Kaplan-Meier survival and log-rank analysis indicated that shorter overall survival was strongly linked to decreased expression of miR-217 and miR-646 (log-rank test P = 0.034, P = 0.026). In terms of miR-217, multivariate Cox proportional hazards model analysis has showed that reduction of miR-217 expression (P = 0.001), TNM stage (P = 0.046), and lymph node metastasis (P = 0.006) were independently linked to a short-time survival of patients. In terms of miR-646, low expression of miR-646 (P = 0.021), TNM stage (P = 0.052), and tumor size (P = 0.043) were independently associated with poor survival of patients as prognostic factors. Our findings suggested that downregulation of MiR-217 and miR-646 was associated with progression of osteosarcoma. MiR-217 and miR-646 may play a key role in suppression of tumor in osteosarcoma and would be applied as a novel therapeutic agent.

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Acknowledgments

We thank Dr. Heydari, Dr. Mousavi and Dr. Alizadeh for their technical assistance in this article.

Conflicts of interest

None

Author information

Authors and Affiliations

  1. Department of Orthopedics Surgery, Islamic Azad University, Tehran Medical Branch, Tehran, Iran

    Afshin Taheri Azam

  2. Department of Orthopaedic and Trauma Surgery, Birjand University of Medical Sciences, Birjand, Iran

    Reza Bahador & Mohammadreza Shakeri

  3. Trauma Research Center, Department of Orthopedics, Baqiyatallah Hospital, Baqiyatallah University of Medical Sciences, Tehran, Iran

    Hamid Hesarikia

  4. Department of Orthopedic Surgery, Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran

    Ali Yeganeh

Authors
  1. Afshin Taheri Azam
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  2. Reza Bahador
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  3. Hamid Hesarikia
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  4. Mohammadreza Shakeri
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  5. Ali Yeganeh
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Corresponding author

Correspondence to Ali Yeganeh.

Additional information

This article has been retracted at the request of the Editor-in-Chief, the International Society of Oncology and BioMarkers (ISOBM) and the Publisher per the Committee on Publication Ethics guidelines. The article shows evidence of irregularities in authorship during the submission process, there is strong reason to believe that the peer review process was compromised and there are similarities with the following articles which were all submitted within a close timeframe:

Masoomeh Dadpay, Mojtaba Zarea, Rahman Ghaffarzadegan Rabati, Bijan Rezakhaniha, Babak Barari, Vahid Behnod, Katayoun Ziari, Upregulation of miR-21 and downregulation of miR-494 may serve as emerging molecular biomarkers for prediagnostic samples of subjects who developed nasopharyngeal carcinoma associates with lymph node metastasis and poor prognosis. Tumor Biol. First Online: 21 August 2015 DOI: 10.1007/s13277-015-3905-1

Date received: 27 June 2015

Seyyed Hasan Karbasy, Afshin Taheriazam, Alireza Mirghasemi, Farnoush Sedaghati, Mohammadreza Shakeri, Emad Yahaghi, Reza Bahador, Upregulation of miR-300 and downregulation of miR-125b act as potential predictor biomarkers in progression, metastasis, and poor prognosis of osteosarcoma. Tumor Biol. First Online: 02 September 2015 DOI: 10.1007/s13277-015-4000-3

Date received: 24 July 2015

The retracted article was received: 5 July 2015.

As such the validity of the content of this article cannot be verified.

An erratum to this article is available at http://dx.doi.org/10.1007/s13277-016-5478-z.

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Azam, A.T., Bahador, R., Hesarikia, H. et al. RETRACTED ARTICLE: Downregulation of microRNA-217 and microRNA-646 acts as potential predictor biomarkers in progression, metastasis, and unfavorable prognosis of human osteosarcoma. Tumor Biol. 37, 5769–5773 (2016). https://doi.org/10.1007/s13277-015-3821-4

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  • DOI: https://doi.org/10.1007/s13277-015-3821-4

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