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Salinomycin radiosensitizes human nasopharyngeal carcinoma cell line CNE-2 to radiation

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Tumor Biology

Abstract

Nasopharyngeal carcinoma (NPC) is primarily treated by chemoradiation. However, how to promote radiation sensitivity in NPC remains a challenge. Salinomycin is potentially useful for the treatment of cancer. This study aimed to explore the radiosensitivity of salinomycin on human nasopharyngeal carcinoma cell line CNE-2. CNE-2 were treated with salinomycin or irradiation, alone or in combination. The cytotoxicity effects of salinomycin were measured using CCK-8 assay. Clonogenic survival assay was used to evaluate the effects of salinomycin on the radiosensitivity of CNE-2. The changes of cell cycle distribution and apoptosis were assayed using flow cytometry. The expression of Caspase3/Bax/Bal-2 was detected by Western blotting. DNA damage was detected via γ-H2AX foci counting. The results showed that salinomycin induced apoptosis and G2/M arrest, increased Bax and cleaved Caspase3, decreased Bcl-2 expression, and increased the formation of γ-H2AX nuclear foci. These data suggest that salinomycin may be a radiosensitizer for NPC radiotherapy.

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Authors and Affiliations

  1. Department of Radiation Oncology, The First People’s Hospital of Zhangjiagang, #68 Jiyangxi Road, Zhangjiagang, Jiangsu province, 215600, China

    Yongqin Zhang, Yun Zuo, Weidong Lu & Zheng Xu

  2. Department of Radiation Oncology, Nantong Tumor Hospital, Affiliated Tumor Hospital of Nantong University, Nantong, 226321, China

    Zhifeng Guan

  3. Department of Radiation Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China

    Hao Zhang, Yan Yang, Meilin Yang, Hongcheng Zhu & Xiaochen Chen

Authors
  1. Yongqin Zhang
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  2. Yun Zuo
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  3. Zhifeng Guan
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  4. Weidong Lu
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  5. Zheng Xu
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  6. Hao Zhang
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  7. Yan Yang
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  8. Meilin Yang
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  9. Hongcheng Zhu
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  10. Xiaochen Chen
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Correspondence to Yun Zuo.

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Zhang, Y., Zuo, Y., Guan, Z. et al. Salinomycin radiosensitizes human nasopharyngeal carcinoma cell line CNE-2 to radiation. Tumor Biol. 37, 305–311 (2016). https://doi.org/10.1007/s13277-015-3730-6

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  • DOI: https://doi.org/10.1007/s13277-015-3730-6

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