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Differentially expressed protein-coding genes and long noncoding RNA in early-stage lung cancer

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Tumor Biology

Abstract

Due to the application of low-dose computed tomography screening, more and more early-stage lung cancers have been diagnosed. Thus, it is essential to characterize the gene expression profile of early-stage lung cancer to develop potential biomarkers for early diagnosis and therapeutic targets. Here, we analyzed microarray data of 181 early-stage lung cancer patients. By comparing gene expression between different tumor and lymph node metastasis stages, we identified various differentially expressed protein-coding genes and long noncoding RNA (lncRNA) in the comparisons of T2 vs. T2 and N1- vs. N0-stage lung cancer. Functional analyses revealed that these differentially expressed genes were enriched in various tumorigenesis or metastasis-related pathways. Survival analysis indicated that two protein-coding genes, C7 and SCN7A, were significantly associated survival of lung cancer. Notably, a novel lncRNA, LINC00313, was highly expressed in both T2- and N1-stage lung cancers. On the other hand, LINC00313 was also upregulated in lung cancer and metastasized lung cancer tissues, compared with adjacent lung tissues and primary lung cancer tissues. Additionally, higher expression level of LINC00313 indicated poor prognosis of lung cancer (hazard ratio = 0.658). Overall, we characterized the expression profiles of protein-coding genes and lncRNA in early-stage lung cancer and found that LINC00313 could be a biomarker for lung cancer.

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Acknowledgments

This study is founded by the Natural Science Foundation of China (81372321 to Lin Xu; 81201830 and 81472200 to Rong Yin), Natural Science Foundation for High Education of Jiangsu Province (13KJB320010 to Rong Yin), Jiangsu Provincial Special Program of Medical Science (BL2012030 to Lin Xu), and Jiangsu Province Ordinary University Graduate Student Research Innovation Project for 2013 (CXLX13_571 to Mantang Qiu).

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    Authors and Affiliations

    1. Department of Thoracic Surgery, Nanjing Medical University Affiliated Cancer Hospital, Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Cancer Institute of Jiangsu Province, Baiziting 42, Nanjing, 210009, China

      Ming Li, Mantang Qiu, Youtao Xu, Qixing Mao, Jie Wang, Gaochao Dong, Wenjia Xia, Rong Yin & Lin Xu

    2. The Fourth Clinical College of Nanjing Medical University, Nanjing, 210000, China

      Mantang Qiu, Qixing Mao & Wenjia Xia

    3. The First Clinical College of Nanjing Medical University, Nanjing, 210000, China

      Youtao Xu

    4. Department of Scientific Research, Nanjing Medical University Affiliated Cancer Hospital, Cancer Institute of Jiangsu Province, Nanjing, 210009, China

      Jie Wang & Gaochao Dong

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    1. Ming Li
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    Correspondence to Rong Yin or Lin Xu.

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    Ming Li and Mantang Qiu contributed equally to this work.

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    Li, M., Qiu, M., Xu, Y. et al. Differentially expressed protein-coding genes and long noncoding RNA in early-stage lung cancer. Tumor Biol. 36, 9969–9978 (2015). https://doi.org/10.1007/s13277-015-3714-6

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    • DOI: https://doi.org/10.1007/s13277-015-3714-6

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