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Japonicone A inhibits the growth of non-small cell lung cancer cells via mitochondria-mediated pathways

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Tumor Biology

Abstract

Japonicone A, which is a natural product isolated from the aerial part of Inula japonica Thunb., has a wide range of clinical applications, including anti-inflammation and anti-oxidation. This study investigated the effects of japonicone A on the growth of non-small cell lung cancer (NSCLC) cell lines. The results showed that japonicone A significantly inhibited the growth of NSCLC cell lines in a dose- and time-dependent manner. This product also blocked cell cycle progression at S phase and induced mitochondrial-related apoptosis by upregulating Bax, cleaved caspase-9, cleaved caspase-3, and cleaved poly(ADP-ribose) polymerase (PARP) protein levels and by downregulating Bcl-2, cyclin D1, CDC25A, and CDK2 protein levels. In vivo, japonicone A suppressed tumor growth via the same mechanism as that observed in vitro. In conclusion, our study is the first to report that japonicone A has an inhibitory effect on the growth of NSCLC cells, indicating that japonicone A administration is a potential therapeutic approach for future NSCLC treatments.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81402403), Shanghai Rising-Star Program (No. 15QA1403100), and the Natural Science Foundation of Shanxi Province (Nos. 20110313013-3 and 2014021037-3).

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    Authors and Affiliations

    1. Department of Respiratory Medicine, Shanxi Medical University Second Hospital, Taiyuan, China

      Yan Du, Jiannan Gong, Xinrui Tian, Xiaomei Yan, Tao Guo, Min Huang, Xiaoyun Hu, Hui Liu, Yinping Wang & Jianqiang Li

    2. Department of General Surgery, Shanxi Medical University Second Hospital, Taiyuan, China

      Bingtai Zhang & Maolan Li

    3. Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China

      Maolan Li

    4. Institute of Biliary Tract Disease, Shanghai Jiao Tong University School of Medicine, Shanghai, China

      Maolan Li

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    1. Yan Du
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    2. Jiannan Gong
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    3. Xinrui Tian
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    4. Xiaomei Yan
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    Correspondence to Jianqiang Li or Maolan Li.

    Additional information

    Maolan Li and Jianqiang Li jointly directed this work.

    Yan Du and Jiannan Gong contributed equally to this work.

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    Du, Y., Gong, J., Tian, X. et al. Japonicone A inhibits the growth of non-small cell lung cancer cells via mitochondria-mediated pathways. Tumor Biol. 36, 7473–7482 (2015). https://doi.org/10.1007/s13277-015-3439-6

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    • DOI: https://doi.org/10.1007/s13277-015-3439-6

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