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Decreased MT1-MMP in gastric cancer suppressed cell migration and invasion via regulating MMPs and EMT

  • Research Article
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Tumor Biology

Abstract

Membrane type 1-matrix metalloproteinase (MT1-MMP) has been identified to play a significant role in several types of cancers, but little is known about the significance of MT1-MMP in gastric cancer patients. The purpose of this study is to investigate the involvement of MT1-MMP in tumor progression of gastric cancer. MT1-MMP expression levels were examined in gastric cancer tissues and cells, and normal gastric tissues and cells. The effects and molecular mechanisms of MT1-MMP expression on cell proliferation, migration, and invasion were also explored. In our results, MT1-MMP messenger RNA (mRNA) and protein expression levels were significantly increased in gastric cancer tissue. Moreover, the overexpression of MT1-MMP was positively associated with the status of clinical stage and lymph node metastasis through real-time PCR. Furthermore, knocking down MT1-MMP expression significantly suppressed the cell migration and invasion in vitro and regulated the expression of MMPs and epithelial-mesenchymal transition (EMT)-associated genes. In conclusions, our study demonstrates that MT1-MMP was overexpressed in gastric cancer tissue, and reduced expression of MT1-MMP suppressed cell migration, invasion, and through regulating the expression of MMPs and the process of EMT in gastric cancer.

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    Authors and Affiliations

    1. Department of Gastrointestinal Surgery of the Eastern Hospital of the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510700, Guangdong, China

      Wenfeng Li, Shouzhi Li, Liang Deng, Shibin Yang, Mingzhe Li, Shuo Long, Sile Chen, Fuxiang Lin & Longbin Xiao

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    1. Wenfeng Li
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    2. Shouzhi Li
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    3. Liang Deng
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    Correspondence to Longbin Xiao.

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    Wenfeng Li and Shouzhi Li are co-first authors.

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    Li, W., Li, S., Deng, L. et al. Decreased MT1-MMP in gastric cancer suppressed cell migration and invasion via regulating MMPs and EMT. Tumor Biol. 36, 6883–6889 (2015). https://doi.org/10.1007/s13277-015-3381-7

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    • DOI: https://doi.org/10.1007/s13277-015-3381-7

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