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RBPJ inhibition impairs the growth of lung cancer

  • Research Article
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Tumor Biology

Abstract

The exact effects of the modulation of Notch signaling pathway on cell growth have been shown to depend on tumor cell type. Recombination signal-binding protein Jκ (RBPJ) is a key transcription factor downstream of receptor activation in Notch signaling pathway. Here, we evaluated the effects of RBPJ inhibition on the growth of lung cancer cells. We found that a short hairpin interfering RNA (shRNA) for RBPJ efficiently inhibited RBPJ expression in lung cancer cells, resulting in a significant decrease in the cell growth. Further analyses showed that RBPJ inhibition altered the levels of its downstream targets, including p21, p27, CDK2, Hes1, Bcl-2, and SKP2, to prevent the cells from growing. Our data thus suggest that shRNA intervention of RBPJ expression could be a promising therapeutic approach for treating human lung cancer.

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Authors and Affiliations

  1. Department of Pulmonary Medicine, The Affiliated Hospital of Hangzhou Normal University, 126 Wenzhou Road, 310015, Hangzhou, China

    Qun Lv, Ronglin Shen & Jianjun Wang

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  1. Qun Lv
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  2. Ronglin Shen
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  3. Jianjun Wang
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Correspondence to Jianjun Wang.

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Qun Lv and Ronglin Shen contribute equally.

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Lv, Q., Shen, R. & Wang, J. RBPJ inhibition impairs the growth of lung cancer. Tumor Biol. 36, 3751–3756 (2015). https://doi.org/10.1007/s13277-014-3015-5

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  • DOI: https://doi.org/10.1007/s13277-014-3015-5

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