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Vascular endothelial growth factor (VEGF) gene polymorphisms and breast cancer risk in Punjabi population from North West India

  • Research Article
  • Published:
Tumor Biology

Abstract

The purpose of this study was to evaluate the association of seven VEGF promoter polymorphisms with breast cancer risk in Punjabi population from North West India. We screened DNA samples of 102 sporadic breast cancer patients and 102 unrelated healthy, gender, and age-matched individuals for seven VEGF promoter polymorphisms [−417C/T (rs833062), −172C/A (rs59260042), −165C/T (rs79469752), −160C/T, −152G/A (rs13207351), −141A/C (rs28357093) and −116G/A (rs1570360)] by direct sequencing. The frequency of GG, GA, and AA genotype of −152G/A polymorphism was 26.47 vs 38.34 %, 46.08 vs 51.96 %, and 27.45 vs 9.80 %, in patients and controls, respectively. VEGF −152 AA genotype was significantly associated with increased risk for breast cancer (OR = 4.04, 95 %CI, 1.69–9.68, p = 0.001; recessive model OR = 3.48, 95 %CI, 1.59–7.63, p = 0.001). For VEGF −116G/A polymorphism, G and A allele frequencies were 65.2 vs 76.47 % and 34.8 vs 23.53 % in patients and controls, respectively. Individuals having −116AA genotype (OR = 3.40; 95 %CI, 1.24–9.37; p = 0.014) and A allele (OR = 1.73; 95 %CI, 1.12–2.67; p = 0.012) were associated with increased risk for breast cancer. VEGF −165C/T and −141A/C polymorphisms were associated with reduced risk for breast cancer. There was significantly decreased frequency of CT genotype (4.90 vs 18.63 %; p = 0.002) and T allele (2.45 vs 9.31 %; p = 0.003) of −165C/T polymorphism among breast cancer patients as compared to controls. VEGF −141A and C allele frequency were 96.57 vs 91.18 % and 3.43 vs 8.82 % in patients and controls, respectively. Significant reduced risk for breast cancer was observed with AC genotype (OR = 0.34, 95 %CI, 0.14–0.86; p = 0.019) and C allele (OR = 0.37; 95 %CI, 0.15–0.89; p = 0.023) of −141A/C polymorphism. We did not observe association of VEGF −417T/C, −172C/A, −160C/T polymorphisms with breast cancer risk in the studied subjects (p > 0.05). The VEGF −152G/A and −116G/A polymorphisms were found to be significantly associated with increased risk for breast cancer while −165C/T and −141A/C polymorphisms were found to be associated with decreased risk for breast cancer in Punjabi population from North West India.

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Abbreviations

VEGF :

Vascular endothelial growth factor

UTR:

Untranslated region

SNP:

Single nucleotide polymorphism

PCR:

Polymerase chain reaction

RFLP:

Restriction fragment length polymorphism

SD:

Standard deviation

HWE:

Hardy-Weinberg Equilibrium

OR:

Odds ratio

CI:

Confidence interval

BMI:

Body mass index

WHR:

Waist to hip ratio

WSR:

Waist to stature ratio

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Acknowledgments

The present study was supported by the DBT grant BT/PR 13252/GBD/27/236/2009 sanctioned to KG and VS. Research fellowship (No.3/1/3/JRF-2012/HRD) to RK from ICMR is duly acknowledged. We are thankful to Dr. Geeta Sharma, Principal, Sri Guru Ram Das Institute of Medical Sciences and Research, Vallah, Amritsar, Punjab for their help in providing access to patients and facilities for execution of research work.

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Authors and Affiliations

  1. Human Cytogenetics Laboratory, Department of Human Genetics, Guru Nanak Dev University, Amritsar, 143005, Punjab, India

    Ruhi Kapahi, Kamlesh Guleria & Vasudha Sambyal

  2. Department of Pathology, Sri Guru Ram Das Institute of Medical Sciences and Research, Amritsar, Punjab, India

    Mridu Manjari

  3. Department of Radiotherapy, Sri Guru Ram Das Institute of Medical Sciences and Research, Amritsar, Punjab, India

    Meena Sudan

  4. Department of Surgery, Sri Guru Ram Das Institute of Medical Sciences and Research, Amritsar, Punjab, India

    Manjit Singh Uppal & Neeti Rajan Singh

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  1. Ruhi Kapahi
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Correspondence to Kamlesh Guleria.

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Kapahi, R., Guleria, K., Sambyal, V. et al. Vascular endothelial growth factor (VEGF) gene polymorphisms and breast cancer risk in Punjabi population from North West India. Tumor Biol. 35, 11171–11181 (2014). https://doi.org/10.1007/s13277-014-2404-0

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