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Krüppel-like factor 5 as potential molecular marker in cervical cancer and the KLF family profile expression

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Tumor Biology

Abstract

Cervical cancer (CC) as other cancer types, presents molecular deregulations, such as the alterations of transcription factors. Krüppel-like factors (KLF) are a family of transcriptional regulators. They are involved in diverse cellular processes, such as proliferation, apoptosis, and angiogenesis among others. Here, we analyzed the expression of all 17 KLF members at messenger RNA (mRNA) level, and protein expression of the two most commonly altered KLF5 and KLF6 in cervical tissues. Fifty-nine cervical tissues ranging from normal tissue to CC were evaluated for KLF1–17 mRNA expression by end-point RT-PCR and KLF5 by qRT-PCR. For KLF5 and KLF6 protein analysis, a tissue microarray was constructed containing the 59 cases and subjected for immunohistochemistry assay and KLF6 IVS1-27G>A single nucleotide polymorphism by direct DNA sequencing. KLF2–16 expressions were present in normal tissue, whereas all 17 were present in Low-Grade Squamous Intraepithelial Lesion, High-Grade-SIL and CC, unrelated to presence of human papillomavirus (HPV). KLF5 mRNA expression gradually increased throughout the subgroups and overexpressed in CC (p = 0.01). KLF5 and KLF6 proteins were immunodetected in all samples. For the KLF6 SNP analysis, 80 % of the CC population analyzed presented GG genotype and the remaining 20 % presented GA genotype (p = 0.491). Our present data show that KLFs expression could not be related to HPV presence, at least at transcriptional level, and KLF5 mRNA overexpression could represent a potential molecular marker for CC; KLF6 SNP has no relation to increased risk of CC.

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Acknowledgments

We would like to thank Universidad Autónoma de Ciudad Juárez for partial support. During this work, the authors DMR, KTP, PRM, MMR and MRE were recipients of a Consejo Nacional de Ciencia y Tecnologia fellowship. This work was a partial fulfillment by DMR for Ph. D. degree at UACJ.

Funding

This work was partially supported by grant 202222 from Fondos Sectoriales Consejo Nacional de Ciencia y Tecnologia-Mexico.

Conflicts of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Laboratorio de Oncología Genómica, Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, CMN-SXXI. IMSS, Av. Cuauhtémoc 330, Col. Doctores, México, D.F., 06720, Mexico

    Daniel Marrero-Rodríguez, Keiko Taniguchi-Ponciano, Pablo Romero-Morelos, Monica Mendoza-Rodríguez, Miriam Rodriguez-Esquivel, Nancy Muñoz-Hernandez, Hugo Arreola-de la Cruz & Mauricio Salcedo

  2. Laboratorio de Biotecnología, Universidad Autonóma de Ciudad Juárez, Ciudad Juárez, Chihuahua, Mexico

    Daniel Marrero-Rodríguez, Florinda Jimenez-Vega & Claudia Vargas-Requena

  3. Servicio de Patología, Hospital de Oncología CMN-SXXI, IMSS, Mexico, Mexico

    Alejandra Mantilla

  4. Clínica de Displasias, Hospital General de México, México, D.F., Mexico

    Guillermo Gomez-Gutierrez, Luis Serna-Reyna & Jorge Ortiz-Leon

  5. Departamento de Biomedicina Molecular, CINVESTAV, México, D.F., Mexico

    Marco Meraz-Rios

  6. División de Laboratorios de Vigilancia e Investigación Epidemiológica, IMSS, México, D.F., Mexico

    Daniel Hernandez & Angeles Hernandez

  7. Clínica de Colposcopia, Jurisdicción Sanitaria II, Servicios de Salud de Chihuahua, Ciudad Juárez, Chihuahua, México

    Cecilia Díaz-Hernández

  8. Unidad de Apoyo a la Investigación, Instituto Nacional de Rehabilitación, SS, México, D.F., Mexico

    Cindy Bandala

Authors
  1. Daniel Marrero-Rodríguez
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  2. Keiko Taniguchi-Ponciano
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  9. Angeles Hernandez
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  10. Guillermo Gomez-Gutierrez
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Correspondence to Mauricio Salcedo.

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Marrero-Rodríguez, D., Taniguchi-Ponciano, K., Jimenez-Vega, F. et al. Krüppel-like factor 5 as potential molecular marker in cervical cancer and the KLF family profile expression. Tumor Biol. 35, 11399–11407 (2014). https://doi.org/10.1007/s13277-014-2380-4

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