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Hsa-miR-1 downregulates long non-coding RNA urothelial cancer associated 1 in bladder cancer

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Tumor Biology

Abstract

MicroRNAs (miRNAs) are known to mainly target protein-coding genes at post-transcriptional level, resulting in mRNA destabilization and/or translational repression. Long non-coding RNAs (lncRNAs) are emerging as a novel set of targets for miRNAs. Here, we report that downregulated hsa-miR-1 and upregulated lncRNA urothelial cancer associated 1 (UCA1) were inversely expressed in bladder cancer. Hsa-miR-1 decreased the expression of UCA1 in bladder cancer cells in an Ago2-slicer-dependent manner. The binding site between UCA1 and hsa-miR-1 was confirmed. Overexpression of hsa-miR-1 inhibited bladder cancer cell growth, induced apoptosis, and decreased cell motility. Knockdown of UCA1 expression phenocopied the effects of upregulation of hsa-miR-1. Transfection of UCA1 expression vector partly reversed the changes caused by transfection of pre-miR-1 plasmids. This study provides evidence for hsa-miR-1 to play tumor suppressive roles via downregulating lncRNA UCA1 in bladder cancer, which may have potential therapeutic significance.

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Acknowledgments

This work was funded by grant from the Basic Research Program of Shenzhen in China (ZDSY20120615154448514).

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Authors and Affiliations

  1. Guangdong Key Laboratory of Male Reproductive Medicine and Genetics, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, 518036, China

    Tiantian Wang, Yuchi Li, Zheguang Lin, Zhimao Jiang & Yaoting Gui

  2. Department of Urology, Peking University Shenzhen Hospital, Shenzhen, 518036, China

    Tiantian Wang

  3. Department of Urology, Shenzhen Second People’s Hospital, Shenzhen, 518035, China

    Jiancheng Yuan & Nenggui Feng

  4. Shantou University Medical College, Shantou, 515000, China

    Jiancheng Yuan, Nenggui Feng & Yuchi Li

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  1. Tiantian Wang
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  2. Jiancheng Yuan
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  3. Nenggui Feng
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  4. Yuchi Li
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  5. Zheguang Lin
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  7. Yaoting Gui
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Correspondence to Yaoting Gui.

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Wang, T., Yuan, J., Feng, N. et al. Hsa-miR-1 downregulates long non-coding RNA urothelial cancer associated 1 in bladder cancer. Tumor Biol. 35, 10075–10084 (2014). https://doi.org/10.1007/s13277-014-2321-2

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  • DOI: https://doi.org/10.1007/s13277-014-2321-2

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