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miR-195 Targets HDGF to inhibit proliferation and invasion of NSCLC cells

  • Research Article
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Tumor Biology

Abstract

Non-small cell lung cancer (NSCLC) is one of the most common causes of cancer-related death worldwide. MicroRNAs (miRNAs) play critical roles in the development and progression of NSCLC. miR-195 acts as a tumor suppressor in several cancers, however, its role in NSCLC is not well understood. Herein, we found that miR-195 was significantly decreased in both NSCLC tissues and cell lines. Forced expression of miR-195 significantly suppressed proliferation, migration, and invasion of NSCLC cells. Hepatoma-derived growth factor (HDGF) was identified as a target of miR-195 in NSCLC cells. Overexpression of HDGF dramatically abolished the tumor suppressive role of miR-195 in NSCLC cells. Our results demonstrated a tumor suppressive role of miR-195 in NSCLC, and suggested a potential therapeutic target for NSCLC.

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References

  1. Siegel R, Ward E, Brawley O, Jemal A. Cancer statistics, 2011: the impact of eliminating socioeconomic and racial disparities on premature cancer deaths. CA: Cancer J Clin. 2011;61:212–36.

    Google Scholar 

  2. van Klaveren RJ. Lung cancer screening. Eur J Cancer. 2011;47 Suppl 3:S147–55.

    Article  PubMed  Google Scholar 

  3. Bouyssou JM, Manier S, Huynh D, Issa S, Roccaro AM, Ghobrial IM. Regulation of microRNAs in cancer metastasis. Biochim Biophys Acta. 1845;2014:255–65.

    Google Scholar 

  4. Zagryazhskaya A, Zhivotovsky B: Mirnas in lung cancer: A link to aging. Ageing research reviews 2014

  5. Zhang W, Bai W, Zhang W: MiR-21 suppresses the anticancer activities of curcumin by targeting pten gene in human non-small cell lung cancer a549 cells. Clinical and translational oncology: official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico 2013

  6. Wan L, Zhang L, Fan K, Wang J: MiR-27b targets limk1 to inhibit growth and invasion of NSCLC cells. Molecular and cellular biochemistry 2014

  7. Zhan M, Qu Q, Wang G, Zhou H. Let-7c sensitizes acquired cisplatin-resistant a549 cells by targeting abcc2 and bcl-xl. Die Pharmazie. 2013;68:955–61.

    CAS  PubMed  Google Scholar 

  8. Molina-Pinelo S, Gutierrez G, Pastor MD, Hergueta M, Moreno-Bueno G, Garcia-Carbonero R, et al. Microrna-dependent regulation of transcription in non-small cell lung cancer. PLoS One. 2014;9:e90524.

    Article  PubMed Central  PubMed  Google Scholar 

  9. Zhang N, Wei X, Xu L. Mir-150 promotes the proliferation of lung cancer cells by targeting p53. FEBS Lett. 2013;587:2346–51.

    Article  CAS  PubMed  Google Scholar 

  10. Ke Y, Zhao W, Xiong J, Cao R. Downregulation of miR-16 promotes growth and motility by targeting HDGF in non-small cell lung cancer cells. FEBS Lett. 2013;587:3153–7.

    Article  CAS  PubMed  Google Scholar 

  11. Lei L, Huang Y, Gong W. Inhibition of mir-92b suppresses nonsmall cell lung cancer cells growth and motility by targeting reck. Mol Cell Biochem. 2014;387:171–6.

    Article  CAS  PubMed  Google Scholar 

  12. Luo Q, Wei C, Li X, Li J, Chen L, Huang Y, et al. Microrna-195-5p is a potential diagnostic and therapeutic target for breast cancer. Oncol Rep. 2014;31:1096–102.

    PubMed Central  CAS  PubMed  Google Scholar 

  13. Furuta M, Kozaki K, Tanimoto K, Tanaka S, Arii S, Shimamura T, et al. The tumor-suppressive mir-497-195 cluster targets multiple cell-cycle regulators in hepatocellular carcinoma. PLoS One. 2013;8:e60155.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  14. Hui W, Yuntao L, Lun L, WenSheng L, ChaoFeng L, HaiYong H, et al. Microrna-195 inhibits the proliferation of human glioma cells by directly targeting cyclin d1 and cyclin e1. PLoS One. 2013;8:e54932.

    Article  PubMed Central  PubMed  Google Scholar 

  15. Mao JH, Zhou RP, Peng AF, Liu ZL, Huang SH, Long XH, et al. Microrna-195 suppresses osteosarcoma cell invasion and migration in vitro by targeting fasn. Oncol Lett. 2012;4:1125–9.

    PubMed Central  CAS  PubMed  Google Scholar 

  16. Qu J, Zhao L, Zhang P, Wang J, Xu N, Mi W, Jiang X, Zhang C, Qu J: Microrna-195 chemosensitizes colon cancer cells to the chemotherapeutic drug doxorubicin by targeting the first binding site of bcl2l2 mRNA. Journal of cellular physiology 2013

  17. Deng H, Guo Y, Song H, Xiao B, Sun W, Liu Z, et al. Microrna-195 and microrna-378 mediate tumor growth suppression by epigenetical regulation in gastric cancer. Gene. 2013;518:351–9.

    Article  CAS  PubMed  Google Scholar 

  18. Yongchun Z, Linwei T, Xicai W, Lianhua Y, Guangqiang Z, Ming Y, Guanjian L, Yujie L, Yunchao H: Microrna-195 inhibits non-small cell lung cancer cell proliferation, migration and invasion by targeting MYB. Cancer letters 2014

  19. Huang JS, Chao CC, Su TL, Yeh SH, Chen DS, Chen CT, et al. Diverse cellular transformation capability of overexpressed genes in human hepatocellular carcinoma. Biochem Biophys Res Commun. 2004;315:950–8.

    Article  CAS  PubMed  Google Scholar 

  20. Mori M, Morishita H, Nakamura H, Matsuoka H, Yoshida K, Kishima Y, et al. Hepatoma-derived growth factor is involved in lung remodeling by stimulating epithelial growth. Am J Respir Cell Mol Biol. 2004;30:459–69.

    Article  CAS  PubMed  Google Scholar 

  21. Chen B, Huang T, Jiang J, Lv L, Li H, Xia S. Mir-141 suppresses proliferation and motility of gastric cancer cells by targeting HDGF. Mol Cell Biochem. 2014;388:211–8.

    Article  CAS  PubMed  Google Scholar 

  22. Zhou Y, Zhou N, Fang W, Huo J. Overexpressed HDGF as an independent prognostic factor is involved in poor prognosis in Chinese patients with liver cancer. Diagn Pathol. 2010;5:58.

    Article  PubMed Central  PubMed  Google Scholar 

  23. Uyama H, Tomita Y, Nakamura H, Nakamori S, Zhang B, Hoshida Y, et al. Hepatoma-derived growth factor is a novel prognostic factor for patients with pancreatic cancer. Clin Cancer Res: Off J Am Assoc Cancer Res. 2006;12:6043–8.

    Article  CAS  Google Scholar 

  24. Zhao WY, Wang Y, An ZJ, Shi CG, Zhu GA, Wang B, et al. Downregulation of miR-497 promotes tumor growth and angiogenesis by targeting HDGF in non-small cell lung cancer. Biochem Biophys Res Commun. 2013;435:466–71.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Thoracic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450000, China

    Haizhou Guo, Weihao Li & Tianliang Zheng

  2. Institute of Nephrology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450000, China

    Zhangsuo Liu

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  1. Haizhou Guo
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  2. Weihao Li
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  3. Tianliang Zheng
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  4. Zhangsuo Liu
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Correspondence to Zhangsuo Liu.

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Guo, H., Li, W., Zheng, T. et al. miR-195 Targets HDGF to inhibit proliferation and invasion of NSCLC cells. Tumor Biol. 35, 8861–8866 (2014). https://doi.org/10.1007/s13277-014-2153-0

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  • DOI: https://doi.org/10.1007/s13277-014-2153-0

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