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Chrebp regulates the transcriptional activity of androgen receptor in prostate cancer

  • Research Article
  • Published:
Tumor Biology

Abstract

Androgen receptor (AR), a member of nuclear hormone receptor, plays an essential role in the initiation and progression of prostate cancer (PCa). In the present study, by way of immunoprecipitation followed by mass spectrometry (IP/MS) system, we found that carbohydrate-responsive element-binding protein (Chrebp), a glucose sensor in normal and cancer cells, interacted with AR in LNCaP cells. The interaction was further confirmed by coimmunoprecipitation analysis. Besides, Chrebp is required for the optimal transcriptional activity of AR in promoting the transcription of the prostate-specific antigen (PSA) promoter and messenger RNA (mRNA) expression. Consistently, knockdown of Chrebp using small interfering RNA (siRNA) in LNCaP cells reduced endogenous PSA levels. Together, our study demonstrates that Chrebp interacts with AR and regulates its transcriptional activity.

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Acknowledgments

This work was supported by Dongfang Zhaoyang Talent Plan (DFZY-3).

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Authors and Affiliations

  1. Department of Urology, East Hospital, Tongji University School of Medicine, Jimo Road 150, 200120, Shanghai, People’s Republic of China

    Xue-Lei Wang, Xiao-Fei Wen, Rong-Bing Li, Guang-Ming Qiu, Ji-Ling Wen & Yue-Min Wang

  2. Shanghai Institute of Materia Medica, Chinese Academy of Sciences, 555 Zu Chong Zhi Road, Shanghai, 201203, People’s Republic of China

    Bin Liu

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  1. Xue-Lei Wang
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  2. Xiao-Fei Wen
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  4. Bin Liu
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  5. Guang-Ming Qiu
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  6. Ji-Ling Wen
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Corresponding author

Correspondence to Xiao-Fei Wen.

Additional information

Xue-Lei Wang and Xiao-Fei Wen contributed equally to this work.

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Supplementary Fig. 1

(A-B) mRNA levels of FASN AND L-PK in LNCaP cells with Chrebp overexpression (A) or knockdown (B) (GIF 247 kb)

High resolution image(TIFF 237 kb)

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Wang, XL., Wen, XF., Li, RB. et al. Chrebp regulates the transcriptional activity of androgen receptor in prostate cancer. Tumor Biol. 35, 8143–8148 (2014). https://doi.org/10.1007/s13277-014-2085-8

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  • DOI: https://doi.org/10.1007/s13277-014-2085-8

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