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Triple-negative and luminal A breast tumors: differential expression of miR-18a-5p, miR-17-5p, and miR-20a-5p

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Tumor Biology

Abstract

New concepts in epigenetics, microRNAs, and gene expression analysis have significantly enhanced knowledge of cancer pathogenesis over the last decade. MicroRNAs (miRNAs) are a class of non-coding RNAs that regulate gene expression by base pairing with target messenger RNAs (mRNAs), resulting in the repression of translation or the degradation of mRNA. To compare the carcinogenic process in tumors with different prognoses, we used real-time RT-PCR to evaluate the miRNA expression profiles of 24 triple-negative breast invasive ductal carcinoma, 20 luminal A breast invasive ductal carcinoma, and 13 normal breast parenchyma controls. We extracted total RNA from tissues fixed in formol and embedded in paraffin (FFPE). Results revealed the upregulation of miR-96-5p (9.35-fold; p = 0.000115), miR-182-5p (7.75-fold; p = 0.000033), miR-7-5p (6.71-fold; p = 0.015626), and miR-21-5p (6.10-fold; p = 0.000000) in tumors group. In addition, the expression of miR-125b-5p (4.49-fold; p = 0.000000) and miR-205-5p (4.36-fold; p = 0.006098) was downregulated. When the expression profiles of triple-negative and luminal A tumors were compared, there was enhanced expression of miR-17-5p (4.27-fold; p = 0.000664), miR-18a-5p (9.68-fold; p = 0.000545), and miR-20a-5 (4.07-fold; p = 0.001487) in the triple-negative tumors compared with luminal A. These data suggest that there is a similar regulation of certain miRNAs in triple-negative and luminal A tumors. However, it is possible that differences in the expression of miR-17-92 cluster will explain the phenotypic differences between these molecular tumor subtypes.

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Acknowledgments

This research was funded by Fundação de Amparo à Pesquisa do Estado de São paulo—FAPESP, São Paulo-SP, Brazil.

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Authors and Affiliations

  1. Laboratório de Ginecologia Molecular e Estrutural—LIM 58, Disciplina de Ginecologia, Faculdade de Medicina, Universidade de São Paulo, Rua Dr Arnaldo, 255, Sala 2031, São Paulo-SP, Brazil

    Carlos Marino Cabral Calvano Filho, Daniele Carvalho Calvano-Mendes, Kátia Cândido Carvalho, Gustavo Arantes Maciel & Edmund Chada Baracat

  2. Setor de Mastologia, Disciplina de Ginecologia, Instituto do Câncer do Estado de São Paulo—ICESP, Faculdade de Medicina, Universidade de São Paulo, Rua Dr Arnaldo, 455, São Paulo-SP, Brazil

    Carlos Marino Cabral Calvano Filho, Daniele Carvalho Calvano-Mendes, Marcos Desidério Ricci, José Roberto Filassi & Edmund Chada Baracat

  3. Fundação Oncocentro de São Paulo, Rua Oscar Freire, 2396, São Paulo-SP, Brazil

    Ana Paula Torres

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  1. Carlos Marino Cabral Calvano Filho
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Correspondence to Daniele Carvalho Calvano-Mendes.

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Calvano Filho, C.M.C., Calvano-Mendes, D.C., Carvalho, K.C. et al. Triple-negative and luminal A breast tumors: differential expression of miR-18a-5p, miR-17-5p, and miR-20a-5p. Tumor Biol. 35, 7733–7741 (2014). https://doi.org/10.1007/s13277-014-2025-7

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  • DOI: https://doi.org/10.1007/s13277-014-2025-7

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