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Long noncoding RNA SPRY4-IT1 is upregulated in esophageal squamous cell carcinoma and associated with poor prognosis

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Tumor Biology

Abstract

LncRNA SPRY4-IT1 has been shown to promote the progression of melanoma. However, the role of lncRNA SPRY4-IT1 in human esophageal squamous cell carcinoma (ESCC) remains unclear. The purpose of this study is to investigate the clinical significance and biological functions of SPRY4-IT1 in ESCC. The expression levels of lncRNA SPRY4-IT in 92 ESCC patients and 8 ESCC cell lines were evaluated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The prognostic significance was evaluated using Kaplan–Meier and Cox regression analyses. Small interfering RNA (siRNA) was used to suppress SPRY4-IT1 expression in ESCC cell lines. Both in vitro and in vivo assays were performed to further explore its role in tumor progression. SPRY4-IT1 levels were significantly higher in ESCC tissues and cells than in corresponding adjacent noncancerous tissues and nontumorigenic esophageal epithelial cells, and the ESCC patients with higher SPRY4-IT1 expression had an advanced clinical stage and poorer prognosis than those with lower SPRY4-IT1 expression. The multivariate analysis revealed that SPRY4-IT1 expression level is an independent prognostic factor in ESCC patients. In vitro assays demonstrated that knockdown of SPRY4-IT1 reduced cell proliferation, invasiveness, and migration. In vivo assays demonstrated that knockdown of SPRY4-IT1 decreases cell growth. SPRY4-IT1 is a novel molecule involved in ESCC progression, which may provide a potential prognostic biomarker and a potential target for therapeutic intervention.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (81201881), the Project of Scientific Education and Vitalizing Health Service for Leading Talents and Innovation Teams from the Ministry of Health and Welfare of Jiangsu Province in 2011 [Jiangsu Health Scientific Education (2011) no. 15], Key Project of Nanjing Medical University (2013NJMU082), and Nanjing City Committee of Science and Technology (201108027).

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Authors and Affiliations

  1. Department of Surgical Oncology, Affiliated Nanjing Hospital of Nanjing Medical University, Nanjing, Jiangsu, China

    Hai-Wei Xie, Bin Zhu, Lv Ji, Su-Qing Li, Chun-Mei Wang, Yu-Suo Tong, Lei Tuo, Jin Lv & Xiu-Feng Cao

  2. Department of Thoracic Surgery, Huaian Hospital Affiliated to Nanjing Medical University, Huaian, Jiangsu, China

    Qing-Quan Wu

  3. Department of Oncology, Changzhou Second Hospital Affiliated to Nanjing Medical University, Changzhou, Jiangsu, China

    Fang-Jun Chen

  4. Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, Jiangsu, China

    Ming Wu

  5. State Key Laboratory of Molecular Oncology, Cancer Institute, Chinese Academy of Medical Sciences, Beijing, China

    Zhi-Hua Liu

  6. Department of Medical Technology, Yancheng Health Vocational and Technical College, Yancheng, Jiangsu, China

    Wei-Hong Shi

Authors
  1. Hai-Wei Xie
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  2. Qing-Quan Wu
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  3. Bin Zhu
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  4. Fang-Jun Chen
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  5. Lv Ji
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  6. Su-Qing Li
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  7. Chun-Mei Wang
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  8. Yu-Suo Tong
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  9. Lei Tuo
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  10. Ming Wu
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  11. Zhi-Hua Liu
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  12. Jin Lv
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  13. Wei-Hong Shi
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  14. Xiu-Feng Cao
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Corresponding authors

Correspondence to Jin Lv, Wei-Hong Shi or Xiu-Feng Cao.

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Xie, HW., Wu, QQ., Zhu, B. et al. Long noncoding RNA SPRY4-IT1 is upregulated in esophageal squamous cell carcinoma and associated with poor prognosis. Tumor Biol. 35, 7743–7754 (2014). https://doi.org/10.1007/s13277-014-2013-y

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  • DOI: https://doi.org/10.1007/s13277-014-2013-y

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