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Genetic variants in NAMPT predict bladder cancer risk and prognosis in individuals from southwest Chinese Han group

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Tumor Biology

Abstract

Nicotinamide phosphoribosyltransferase (Nampt) was served as a useful biomarker for tumorigenesis and for the prediction of cancer survival. In the present study, we analyzed the SNPs of the NAMPT gene and their impact on the susceptibility and prognosis for patients with bladder cancer (BC). The rs61330082, rs2505568 and rs9034 were selected and genotyped by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) method in 407 patients with bladder cancer and 316 ethnicity-matched healthy control subjects. The genotyping method was confirmed by the DNA sequencing analysis. Statistically significant increased bladder cancer risk was found to be associated with the C allele and CC genotype of rs61330082; nevertheless, decreased bladder cancer risk was revealed to be associated with A allele and AT genotype of rs2505568. Stratified analyses revealed the rs61330082 to be statistically associated with increased bladder cancer risk in smokers and increased invasiveness of bladder cancer. The AT heterozygote of rs2505568 may prevent the recurrence of bladder cancer. Kaplan-Meier curves revealed a statistically significant association of rs2505568 with recurrence-free survival for total bladder cancer patients and non-muscle-invasive bladder cancer patients, and a statistically significant association of rs9034 with recurrence-free survival for muscle-invasive bladder cancer patients. Multiple Cox regression analysis identified the rs2505568 as a possible independent prognostic factor for recurrence-free survival in total bladder cancer patients. Our results suggested an important role for NAMPT in the pathogenesis of bladder cancer and SNPs of NAMPT gene might be a novel genetic biomarker for the prognosis of bladder cancer.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (no. 81172440, no. 81172494, and no. 81272821), the Applied Basic Research Programs of Science and Technology Commission Foundation of Sichuan Province (no. 2012SZ0008), and Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT0935).

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Authors and Affiliations

  1. Department of Forensic Pathology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, China

    Kui Zhang

  2. Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu, 610041, China

    Kui Zhang, Peng Chen, Yan Pu & Lin Zhang

  3. Laboratory of Molecular Translational Medicine, West China Institute of Women and Children’s Health, West China Second University Hospital, Sichuan University, Chengdu, China

    Bin Zhou, Yaping Song & Lin Zhang

  4. Department of Urology, West China Hospital, Sichuan University, Chengdu, China

    Peng Zhang

  5. Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, China

    Zhu Zhang

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  1. Kui Zhang
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  2. Bin Zhou
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  3. Peng Zhang
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  4. Zhu Zhang
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  8. Lin Zhang
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Correspondence to Lin Zhang.

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Kui Zhang and Bin Zhou contribute equally to this work.

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Zhang, K., Zhou, B., Zhang, P. et al. Genetic variants in NAMPT predict bladder cancer risk and prognosis in individuals from southwest Chinese Han group. Tumor Biol. 35, 4031–4040 (2014). https://doi.org/10.1007/s13277-013-1527-z

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  • DOI: https://doi.org/10.1007/s13277-013-1527-z

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