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Tumour necrosis factor alpha 308 G/A is a risk marker for the progression from high-grade lesions to invasive cervical cancer

  • Research Article
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Tumor Biology

Abstract

Tumour necrosis factor alpha (TNF-α) is a strong pro-inflammatory cytokine with important functions on immune response to viral infections. A single nucleotide polymorphism on the −308 position of the promoter region of TNFA gene characterised by a G > A transition has been associated with different TNF-α levels and therefore with differential susceptibility for the development several diseases. A cross-sectional case–control study was performed with 509 women with cancer from the northern region of Portugal, including 205 healthy women and 337 women with different cervical lesions including invasive cervical. The −308G > A polymorphism genotyping was performed with TaqMan® SNP Genotyping Assay and studied for its association with cervical cancer development. This study showed increased frequency of the −308A allele in women with any cervical lesions. Statistical analysis revealed that −308A carriers are associated with an almost 2-fold increased risk for invasive cervical cancer development (p = 0.005; odds ratio (OR) = 1.87). Similar results were found when comparing the risk of progression between preinvasive lesions and invasive cervical cancer development (p = 0.002; OR = 2.41). Our results reveal that −308 TNFA AA individuals are at increased risk of invasive cervical cancer development and more important, that the risk is significantly increased for the progression from premalignant lesion to invasive cancer. Considering previous data and this study, this polymorphism confirms to be a significant marker in our population.

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Acknowledgments

This study was performed as part of the Doctoral Degree of the first author which was awarded with an individual grant for Doctoral degree by Fundação para a Ciência e Tecnologia (SFRH/BD/40718/2007). Authors also would like to acknowledge to the Portuguese League Against Cancer (Liga Portuguesa Contra o Cancro—Núcleo Regional do Norte) for the support to the Molecular Oncology Group.

Conflicts of interest

None

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Authors and Affiliations

  1. Molecular Oncology Group, Portuguese Institute of Oncology of Porto, Rua Dr. António Bernardino Almeida, 4200-072, Porto, Portugal

    Hugo Sousa, Sara Oliveira, Alexandra M. Santos, Raquel Catarino & Rui Medeiros

  2. Molecular Virology Laboratory of Virology Service, Portuguese Institute of Oncology of Porto, Rua Dr. António Bernardino Almeida, 4200-072, Porto, Portugal

    Hugo Sousa & Rui Medeiros

  3. ICBAS, Abel Salazar Institute for the Biomedical Sciences, University of Porto, Porto, Portugal

    Hugo Sousa, Raquel Catarino & Rui Medeiros

  4. Gynaecological Service, Portuguese Institute of Oncology of Porto, Rua Dr. António Bernardino Almeida, 4200-072, Porto, Portugal

    José Moutinho

  5. Research Department—Portuguese League Against Cancer (LPCC-NRNorte), Estrada Interior da Circunvalação, 6657, 4200-177, Porto, Portugal

    Rui Medeiros

  6. Grupo de Oncologia Molecular—CI, Laboratórios 4º Piso, Instituto Português de Oncologia do Porto FG, EPE, Rua Dr. António Bernardino Almeida, 4200-072, Porto, Portugal

    Hugo Sousa

Authors
  1. Hugo Sousa
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  2. Sara Oliveira
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  3. Alexandra M. Santos
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  4. Raquel Catarino
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  5. José Moutinho
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  6. Rui Medeiros
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Corresponding author

Correspondence to Hugo Sousa.

Additional information

José Moutinho (MD) is actually retired from the institution, but at the date of study development, he was responsible for patient selection.

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Sousa, H., Oliveira, S., Santos, A.M. et al. Tumour necrosis factor alpha 308 G/A is a risk marker for the progression from high-grade lesions to invasive cervical cancer. Tumor Biol. 35, 2561–2564 (2014). https://doi.org/10.1007/s13277-013-1337-3

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  • DOI: https://doi.org/10.1007/s13277-013-1337-3

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