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Effect of the cytokine levels in serum on osteosarcoma

  • Research Article
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Tumor Biology

Abstract

Osteosarcoma (OS) is the most common malignant bone tumor in patients under 20 years old. Studies have shown that cytokines play important roles in regulating immune responses in OS. In the current study, we investigated the effect of cytokines on OS by assessing serum cytokine profiles. Serum levels of 11 cytokines were measured by multiplex protein arrays in 58 patients with OS and 72 healthy controls. Results showed that serum levels of interleukin 1 receptor antagonist (IL-1Ra), IL-6, IL-8, and tumor necrosis factor-alpha (TNF-α) were significantly increased in patients than in controls (2.5-fold, 2.4-fold, 2.7-fold, and 2.1-fold, respectively). When comparing the expression of cytokines in OS patients with different clinical parameters, cases with osteoblastic subtype revealed increased level of IL-6 than patients with other subtypes (p < 0.05); cases with metastasis demonstrated significantly higher level of TNF-α than those without metastasis (p < 0.05), whereas OS patients whose tumor size were bigger than 8 cm presented elevated levels of IL-8 and TNF-α than those with small tumor size (p < 0.05 and p < 0.05, respectively). These data indicated that IL-1Ra, IL-6, IL-8, and TNF-α were associated with increased risk of OS, in which IL-8 and TNF-α may be further correlated with the progression of this disease.

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No competing financial interests exist.

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Authors and Affiliations

  1. Department of Orthopedics, 324th Hospital of the People’s Liberation Army, Chongqing, China

    Hong Xiao

  2. Department of Gastroenterology, 324th Hospital of the People’s Liberation Army, Chongqing, China

    Ling Chen

  3. Department of Orthopedics, Xinqiao Hospital, Third Military Medical University, 83 Xinqiao Road, Chongqing, 400037, China

    Gang Luo & Wenjie Zheng

  4. University of Toronto, Toronto, ON, Canada

    Haihang Son & James H. Prectoni

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  1. Hong Xiao
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  2. Ling Chen
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  3. Gang Luo
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  4. Haihang Son
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  6. Wenjie Zheng
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Corresponding author

Correspondence to Wenjie Zheng.

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Hong Xiao and Ling Chen contributed equally to this work.

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Xiao, H., Chen, L., Luo, G. et al. Effect of the cytokine levels in serum on osteosarcoma. Tumor Biol. 35, 1023–1028 (2014). https://doi.org/10.1007/s13277-013-1136-x

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  • DOI: https://doi.org/10.1007/s13277-013-1136-x

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