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Association of Kaposi’s sarcoma-associated herpesvirus (KSHV) with bladder cancer in Croatian patients

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Tumor Biology

Abstract

As the seventh most common human malignancy, bladder cancer represents a global health problem. In addition to well-recognized risk factors such as smoking and exposure to chemicals, various infectious agents have been implicated as cofactors in the pathogenesis of urothelial malignancies. The aim of the present study was to assess the possible association of viral infection and bladder cancer in Croatian patients. Biopsy specimens were collected from a total of 55 patients diagnosed with different stages of bladder cancer. Initial screening of DNA extracts for the presence of viruses on Lawrence Livermore Microbial Detection Array revealed Kaposi’s sarcoma-associated herpesvirus (KSHV) in each of three randomly chosen biopsy specimens. The prevalence of infection with KSHV among study population was then examined by KSHV-specific polymerase chain reaction (PCR) and immunoblotting. By nested PCR, KSHV DNA was detected in 55 % of patients. KSHV, also known as human herpesvirus 8, is an infectious agent known to cause cancer. Its oncogenic potential is primarily recognized from its role in Kaposi’s sarcoma, but it has also been involved in pathogenesis of two lymphoproliferative disorders. A high prevalence of KSHV infection in our study indicates that KSHV may play a role in tumorigenesis of bladder cancer and warrants further studies.

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Acknowledgments

The authors want to thank to Mrs. Sandra Vujević for excellent technical assistance during sample preparation and to Mr. James Thissen from Lawrence Livermore National Laboratory for his technical assistance in microarray experiments. We thank Prof. Ivan Đikić for providing us with many reagents needed for this study. This project was supported by the Croatian Ministry of Science, Education and Sports, grant number 216-000000-3348 to JT and grant number 216-0481153-1148 to VPP and by the city of Split support to JT laboratory.

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None.

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Authors and Affiliations

  1. School of Medicine, University of Split, Šoltanska 2, 21000, Split, Croatia

    Martina Paradžik, Marina Degoricija, Volga Punda-Polić & Janoš Terzić

  2. Department of Chemistry, Faculty of Science, University of Split, N. Tesle 12, 21000, Split, Croatia

    Viljemka Bučević-Popović

  3. Department of Urology, Clinical Hospital Split, Šoltanska 2, 21000, Split, Croatia

    Marijan Šitum

  4. Global Security, Lawrence Livermore National Laboratory, Livermore, CA, 94551, USA

    Crystal J. Jaing & Kevin S. McLoughlin

  5. Molecular Biology Research Lab, Department of Biochemistry, Faculty of Medicine, University of Jordan, Amman, 11942, Jordan

    Said I. Ismail

Authors
  1. Martina Paradžik
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  2. Viljemka Bučević-Popović
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  8. Volga Punda-Polić
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  9. Janoš Terzić
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Correspondence to Janoš Terzić.

Additional information

Martina Paradžik, Viljemka Bučević-Popović, and Marijan Šitum have equally contributed to this work.

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Paradžik, M., Bučević-Popović, V., Šitum, M. et al. Association of Kaposi’s sarcoma-associated herpesvirus (KSHV) with bladder cancer in Croatian patients. Tumor Biol. 35, 567–572 (2014). https://doi.org/10.1007/s13277-013-1079-2

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  • DOI: https://doi.org/10.1007/s13277-013-1079-2

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