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Tropomyosin-4 correlates with higher SBR grades and tubular differentiation in infiltrating ductal breast carcinomas: an immunohistochemical and proteomics-based study

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Tumor Biology

Abstract

The aim of this study is to evaluate tropomyosin-4 (TM4) expression in infiltrating ductal breast carcinomas (IDCAs), as well as its prognostic significance. Using a 2-DE/MALDI-TOF mass spectrometry investigation coupled with an immunohistochemical approach, we have assessed the expression of TM4 in IDCAs, as well as in other types of breast tumors. Proteomic analyses revealed an increased expression of tropomyosin-4 in IDCA tumors. Using immunohistochemistry, overexpression of tropomyosin-4 was confirmed in 51 additional tumor specimens. Statistical analyses revealed, however, no significant correlations between tropomyosin-4 expression and clinicopathological parameters of the disease including tumor stage, patient age, estrogen and progesterone receptor status, and lymph node metastasis occurrence. A significant association was found, however, with a high Scarf–Bloom–Richardson (SBR) grade, a known marker of tumor severity. Additionally, the SBR component showing a correlation with TM4 expression was the tubular differentiation status. This study demonstrates the upregulation of tropomyosin-4 in IDCA tissues, which may highlight its involvement in breast cancer development. Our findings also support a link between tropomyosin-4 expression and aggressiveness of IDCA tumors.

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Acknowledgments

This work was supported by le Ministère de l’Enseignement Supérieur et de la Recherche Scientifique, le Ministère de la Santé Publique de la République Tunisienne, and by the Center National de Recherche Scientifique (Strasbourg, France).

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Authors and Affiliations

  1. Laboratoire d’Immuno-Oncologie Moléculaire, Faculté de Médecine de Monastir, Al Munastir, Tunisia

    Maria Kabbage & Karim Chahed

  2. Département de Pathologie, Center Hospitalo-Universitaire-Farhat-Hached, Sousse, Tunisia

    Mounir Trimeche

  3. Institut Supérieur des Sciences Appliquées et Technologiques ISSAT, Mahdia, Tunisia

    Hela ben Nasr

  4. Plate Forme Protéomique, Institut de Biologie Moléculaire et Cellulaire, CNRS, 67084, Strasbourg, France

    Philippe Hammann & Lauriane Kuhn

  5. Institut Supérieur de Biotechnologie de Monastir, Monastir, Tunisia

    Bechr Hamrita

  6. Faculté des Sciences de Sfax, Université de Sfax, Sfax, Tunisia

    Karim Chahed

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  1. Maria Kabbage
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  2. Mounir Trimeche
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  3. Hela ben Nasr
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  4. Philippe Hammann
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  5. Lauriane Kuhn
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  7. Karim Chahed
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Correspondence to Karim Chahed.

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Kabbage, M., Trimeche, M., ben Nasr, H. et al. Tropomyosin-4 correlates with higher SBR grades and tubular differentiation in infiltrating ductal breast carcinomas: an immunohistochemical and proteomics-based study. Tumor Biol. 34, 3593–3602 (2013). https://doi.org/10.1007/s13277-013-0939-0

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  • DOI: https://doi.org/10.1007/s13277-013-0939-0

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