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Association of peripheral CD4+ CXCR5+ T cells with chronic lymphocytic leukemia

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Tumor Biology

Abstract

Accumulating evidences indicate that immune dysregulation plays a key role in both lymphomagenesis and patient outcome of chronic lymphocytic leukemia (CLL). Peripheral blood CD4+ CXCR5+ T cells, known as circulating follicular helper T cells (Tfh), can induce B cell activation and production of specific antibody responses. The aim of the study was to investigate changes of circulating Tfh in CLL. Tfh and it subtypes were tested by measuring CD4, CXCR5, CXCR3, and CCR6 in 72 CLL cases and 86 healthy controls using flow cytometry. Data showed that the percentage of Tfh in the peripheral CD4+ T cells was significantly increased in CLL (25.1 %) than in controls (8.4 %) (p < 0.001). Further analysis revealed that the upregulation of Tfh was contributed by Tfh-th2 subtype and Tfh-th17 subtype. Investigating staging of the cases demonstrated that the prevalence of Tfh was significantly elevated in cases with Binet stage C (37.3 %) than those with stage A (20.1 %) or stage B (23.9 %). In addition, we analyzed Tfh in patients with immunoglobulin variable heavy chain (IGHV) gene mutational status. Results presented that Tfh-th17 subtype had clearly higher frequency in patients with IGHV mutation compared to the unmutated cases (p = 0.035). This study suggested the involvement of Tfh in the pathogenesis and progression of CLL, and provided a potential target for treating this disease.

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Acknowledgments

This study was supported by the Public Health Project of the Shanghai Health Bureau (no. 12GWZX0202) and Changhai Hospital 1255 Project (no. CH125541100).

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Authors and Affiliations

  1. Department of Transfusion, Changhai Hospital, Second Military Medical University, 168 Changhai Road, Shanghai, 200433, China

    Zhanshan Cha, Yan Zang, Huijun Guo, Haihui Gu, Xiaohua Tu & Baohua Qian

  2. The University of Texas MD Anderson Cancer Center, Houston, TX, 77030, USA

    James R. Rechlic & Lindsay M. Olasnova

  3. Department of Internal Medicine, Emergency Center, Shanghai East Hospital, Tongji University, 150 Jimo Road, Shanghai, 200120, China

    Haihan Song

Authors
  1. Zhanshan Cha
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  2. Yan Zang
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  3. Huijun Guo
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  4. James R. Rechlic
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  5. Lindsay M. Olasnova
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  6. Haihui Gu
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  7. Xiaohua Tu
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  8. Haihan Song
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  9. Baohua Qian
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Corresponding authors

Correspondence to Haihan Song or Baohua Qian.

Additional information

Zhanshan Cha and Yan Zang contributed equally to this work.

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Cha, Z., Zang, Y., Guo, H. et al. Association of peripheral CD4+ CXCR5+ T cells with chronic lymphocytic leukemia. Tumor Biol. 34, 3579–3585 (2013). https://doi.org/10.1007/s13277-013-0937-2

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  • DOI: https://doi.org/10.1007/s13277-013-0937-2

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