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Upregulated hPuf-A promotes breast cancer tumorigenesis

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Tumor Biology

Abstract

hPuf-A is a member of RNA-binding PUF family that regulates mRNA translation. Redistribution of hPuf-A from the nucleolus to the nucleoplasm upon genotoxic stress modulates the poly(ADP-ribosyl)ation activity of PARP-1. Here, we report a novel function of hPuf-A involved in promoting breast cancer progression. Immunohistochemical studies showed higher expression levels of hPuf-A in stage I, II, III, and IV breast cancer specimens in contrast with those of hPuf-A in ductal carcinoma in situ. The presence of hPuf-A is highly associated with colony formation capacities in breast cancer T47D and MDA-MB-231 cells. Xenograft growth of hPuf-A-silenced and hPuf-A overexpressing MDA-MB-231 cells in nude mice was substantially in concert with colony formation capacities. This promoting effect of hPuf-A in tumorigenesis might be correlated with the regulation of its associated mRNAs, such as RbAp48 and DDX3. Collectively, hPuf-A may have diagnostic values in breast cancer progression.

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Acknowledgments

We are grateful to the staffs of the TC5 Bio-Image Tools, College of Life Science, National Taiwan University, for the microscopic assistance. We thank the technical services provided by Dr. Sheng-Wei Lin at IBC, Academia Sinica. This work was partly supported by the National Science Council of Taiwan (NSC 101-2311-B-002-008) and National Taiwan University (10R70602B3) to MSC.

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Authors and Affiliations

  1. Department of Physiology, Mackay Memorial Hospital, Taipei, Taiwan

    Chi-Chen Fan

  2. Institute of Biochemical Sciences, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617, Taiwan

    Liek-Yeow Lee, Ming-Ying Yu & Mau-Sun Chang

  3. Department of Pathology, Mackay Memorial Hospital, Taipei, Taiwan

    Chi-Yuan Tzen & Chi Chou

  4. Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan

    Mau-Sun Chang

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  1. Chi-Chen Fan
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  2. Liek-Yeow Lee
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  3. Ming-Ying Yu
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  5. Chi Chou
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Correspondence to Mau-Sun Chang.

Additional information

Chi-Chen Fan and Liek-Yeow Lee contributed equally to this study.

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Fan, CC., Lee, LY., Yu, MY. et al. Upregulated hPuf-A promotes breast cancer tumorigenesis. Tumor Biol. 34, 2557–2564 (2013). https://doi.org/10.1007/s13277-013-0801-4

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  • DOI: https://doi.org/10.1007/s13277-013-0801-4

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