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Lentivirus-delivered Krüppel-like factor 8 small interfering RNA inhibits gastric cancer cell growth in vitro and in vivo

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Tumor Biology

Abstract

We found that the transcription factor Krüppel-like factor 8 (KLF8) was highly expressed in gastric cancer tissues and cell lines compared with adjacent noncancerous regions and gastric epithelial mucosa cells. We employed a lentivirus-mediated RNAi technique to knockdown KLF8 expression in gastric cancer cell line SGC7901 and observed its effects on cell growth in vitro and in vivo. Knockdown of KLF8 inhibited SGC7901 cell proliferation, promoted cell apoptosis, inhibited the tumorigenicity of SGC7901 cells, and significantly decreased tumor growth when the cells were injected into nude mice. These results indicated that overexpression of KLF8 may influence the biological behavior of SGC7901 gastric cancer cells. Knockdown of KLF8 expression by lentivirus-delivered siRNA may be useful as a therapeutic agent for the treatment of gastric cancer.

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Acknowledgement

This work was supported by the National Foundation of Natural Sciences, China (Number: 30971338).

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Authors and Affiliations

  1. Department of Oncology, Tangdu Hospital, Fourth Military Medical University, 1 Xinsi Road, Xi’an, 710038, China

    Lili Liu, Na Liu, Yi Liu, Jie Min, Hailin Pang, Ning Zhang & Helong Zhang

  2. Departments of Pathology and Pathophysiology, Fourth Military Medical University, 17 West Changle Road, Xi’an, 710032, China

    Min Xu

  3. State Key Laboratory of Cancer Biology, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032, China

    Hongbo Zhang

Authors
  1. Lili Liu
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  2. Na Liu
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  3. Min Xu
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  4. Yi Liu
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  5. Jie Min
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  6. Hailin Pang
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  7. Ning Zhang
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  8. Hongbo Zhang
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  9. Helong Zhang
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Corresponding authors

Correspondence to Hongbo Zhang or Helong Zhang.

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Lili Liu and Na Liu contributed equally to this work.

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Liu, L., Liu, N., Xu, M. et al. Lentivirus-delivered Krüppel-like factor 8 small interfering RNA inhibits gastric cancer cell growth in vitro and in vivo. Tumor Biol. 33, 53–61 (2012). https://doi.org/10.1007/s13277-011-0245-7

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  • DOI: https://doi.org/10.1007/s13277-011-0245-7

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