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RGS16 and FosB underexpressed in pancreatic cancer with lymph node metastasis promote tumor progression

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Tumor Biology

Abstract

Lymph node (LN) metastasis is one of the most important adverse prognostic factors for pancreatic cancer. The aim of this study was to identify novel lymphatic metastasis-associated markers for pancreatic cancer. DNA microarray analysis was used to determine and compare the expression profiles of 17 pancreatic cancer tissues with LN metastasis and 17 pancreatic cancer tissues without LN metastasis. The microarray results were validated by real-time reverse transcription-polymerase chain reaction and immunohistochemistry. Only 58 genes were differentially expressed between the two groups with a difference in signal intensity ratio greater than a 1.5-fold change. Of these genes, 30 were significantly down-regulated in the LN metastasis group. Among five selected down-regulated genes for validation using real-time PCR, the expression of DST, FosB, RGS16, and CXCL12 was significantly lower in the LN metastasis group. Immunohistochemical analysis confirmed RGS16 and FosB underexpression in pancreatic cancer tissues with LN metastasis. RGS16 and FosB underexpression was associated with poor patient survival. Our findings show that RGS16 and FosB are underexpressed in pancreatic cancer with lymph node metastasis and associated with reduced survival, suggesting that RGS16 and FosB might be prognostic markers for pancreatic cancer.

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Authors and Affiliations

  1. Health Promotion Center, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea

    Ji Hyang Kim

  2. Samsung Biomedical Research Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea

    Jin Young Lee

  3. Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea

    Kyu Taek Lee, Jong Kyoon Lee, Kwang Hyuck Lee & Jong Chul Rhee

  4. Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea

    Kee-Taek Jang

  5. Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea

    Jin Seok Heo & Seong Ho Choi

  6. Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Irwon-dong, Gangnam-gu, Seoul, South Korea, 135-710

    Kyu Taek Lee

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  1. Ji Hyang Kim
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Correspondence to Kyu Taek Lee.

Additional information

This work was supported by the Samsung Biomedical Research Institute grant # SBRI C-A9-226-1.

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Kim, J.H., Lee, J.Y., Lee, K.T. et al. RGS16 and FosB underexpressed in pancreatic cancer with lymph node metastasis promote tumor progression. Tumor Biol. 31, 541–548 (2010). https://doi.org/10.1007/s13277-010-0067-z

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  • DOI: https://doi.org/10.1007/s13277-010-0067-z

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