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Expression of sphingosine-1-phosphate receptors and lysophosphatidic acid receptors on cultured and xenografted human colon, breast, melanoma, and lung tumor cells

  • Research Article
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Tumor Biology

Abstract

The lysophospholipids sphingosine-1-phosphate (S1P) and lysophosphatidic acid (LPA) are small lipid molecules with a variety of physiological roles. Additionally, their involvement in the initiation and progression of malignant tumors has been increasingly recognized in recent years. However, the data on the expression of S1P and LPA receptors on different cancer cells are very few. Real-time polymerase chain reaction was used for the analysis of mRNA expression of five S1P(1–5) and three LPA(1–3) receptors on a large panel of 13 colon, breast, melanoma, and lung cancer cell lines. Furthermore, the modulation of S1P and LPA receptor mRNA expression was studied upon xenotransplantation of tumor cells into severe combined immunodeficient (SCID) mice. The S1P and LPA receptors were expressed to a variable degree on all tumor cell lines tested (with exception of colon cancer SW480). Most notably, tumor cell lines in vitro expressed S1P2 mRNA that was down-regulated upon xenotransplantation, whereas LPA2 receptor mRNA was strongly expressed both in vitro and in vivo (except by breast cancer cells). The latter was especially distinctive for small cell lung tumor cells. The S1P and LPA receptors are differentially expressed on tumor cell lines in vitro. Their expression is modulated upon xenografting into SCID mice in vivo.

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Acknowledgment

This work was supported by the Ministry of Science and Technological Development of Republic of Serbia (grant no. 145016). Novica M. Milićević was supported by Alexander von Humboldt-Foundation, Bonn, Germany.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Center for Experimental Medicine, Institute of Anatomy II: Experimental Morphology, University Hospital Hamburg-Eppendorf, Hamburg, Germany

    Reinhard Müller, Christoph Berliner, Jessica Leptin, Daniel Pörtner, Wojciech Bialecki & Udo Schumacher

  2. Institute of Nutritional Science, University of Potsdam, Potsdam, Germany

    Burkhard Kleuser

  3. Institute of Histology and Embryology, Faculty of Medicine, University of Beograd, Višegradska 26, 11000, Belgrade, Serbia

    Novica M. Milićević

Authors
  1. Reinhard Müller
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  2. Christoph Berliner
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  3. Jessica Leptin
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  4. Daniel Pörtner
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  5. Wojciech Bialecki
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  6. Burkhard Kleuser
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  7. Udo Schumacher
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  8. Novica M. Milićević
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Correspondence to Novica M. Milićević.

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Müller, R., Berliner, C., Leptin, J. et al. Expression of sphingosine-1-phosphate receptors and lysophosphatidic acid receptors on cultured and xenografted human colon, breast, melanoma, and lung tumor cells. Tumor Biol. 31, 341–349 (2010). https://doi.org/10.1007/s13277-010-0043-7

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  • DOI: https://doi.org/10.1007/s13277-010-0043-7

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