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Forensic and population genetic analyses of the GlobalFiler STR loci in the Mongolian population

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Abstract

We have analyzed 24 loci including autosomal and Y-chromosomal short tandem repeats (STRs), Y-indel, and sex-determining marker in a sample of 267 unrelated individuals from the Mongolian population using the GlobalFiler™ PCR Amplification Kit to provide an expanded and more reliable forensic database. Khalkh among 15 Mongolian minor-groups accounts for about 80% of the entire Mongolian population. A total of 267 different DNA profiles were found in this work. The highest gene diversity was observed in the SE33 (0.9376) locus, and the lowest value was found in the TPOX (0.6142) locus. Although individual power of discrimination estimates varied at the studied loci, combined probability of match from the 21 STR loci was estimated to be 1.139 × 10−24, which is highly informative. Based on the results of pairwise F ST genetic distances and multi-dimensional scaling plot showed that Mongolians were clustered into Europeans and Asians, although Mongolia is geographically located in Northeastern Asia. Thus, the present survey of the Mongolian population may help establish a comprehensive reference database for forensic and population genetic analyses.

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References

  • Allen M, Engström AS, Meyers S, Handt O, Saldeen T, von Haeseler A, Pääbo S, Gyllensten U (1998) Mitochondrial DNA sequencing of shed hairs and saliva on robbery caps: sensitivity and matching probabilities. J Forensic Sci 43:453–464

    Article  CAS  PubMed  Google Scholar 

  • Almeida C, Ribeiro T, Oliveira AR, Porto MJ, Costa Santos J, Dias D, Dario P (2015) Population data of the GlobalFiler® Express loci in South Portuguese population. Forensic Sci Int Genet 19:39–41

    Article  CAS  PubMed  Google Scholar 

  • Babiker HM, Schlebusch CM, Hassan HY, Jakobsson M (2011) Genetic variation and population structure of Sudanese populations as indicated by 15 Identifiler sequence-tagged repeat (STR) loci. Invest Genet 2:12

    Article  Google Scholar 

  • Bacher JW, Helms C, Donis-Keller H, Hennes L, Nassif N, Schumm JW (2000) Chromosome localization of CODIS loci and new pentanucleotide repeat loci. In: Sensabaugh GF, Lincoln PJ, Olaisen B (eds) Progress in forensic genetics 8. Elsevier, New York, pp 33–36

    Google Scholar 

  • Chaudhari RR, Dahiya MS (2014) Genetic diversity of 15 autosomal short tandem repeats loci using the AmpFlSTR® Indentifiler™ kit in a Bhil Tribe Population from Gujarat state, India. Indian J Hum Genet 20:148–152

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • de Knijff P, Kayser M, Caglià A, Corach D, Fretwell N, Gehrig C, Graziosi G, Heidorn F, Herrmann S, Herzog B, Hidding M, Honda K, Jobling M, Krawczak M, Leim K, Meuser S, Meyer E, Oesterreich W, Pandya A, Parson W, Penacino G, Perez-Lezaun A, Piccinini A, Prinz M, Roewer L (1997) Chromosome Y microsatellites: population genetic and evolutionary aspects. Int J Legal Med 110:134–149

    Article  PubMed  Google Scholar 

  • Dobashi Y, Kido A, Fujitani N, Hara M, Susukida R, Oya M (2005) STR data for the AmpFLSTR Identifiler loci in Bangladeshi and Indonesian populations. Leg Med 7:222–226

    Article  CAS  Google Scholar 

  • Edwards A, Hammond HA, Jin L, Caskey CT, Chakraborty R (1992) Genetic variation at five trimeric and tetrameric tandem repeat loci in four human population groups. Genomics 12:241–253

    Article  CAS  PubMed  Google Scholar 

  • Excoffier L, Lischer HE (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567

    Article  PubMed  Google Scholar 

  • Felsenstein J (2013) PHYLIP version 3.695. Department of Genome Sciences, University of Washington, Seattle

  • Forster P, Röhl A, Lünnemann P, Brinkmann C, Zerjal T, Tyler-Smith C, Brinkmann B (2000) A short tandem repeat-based phylogeny for the human Y chromosome. Am J Hum Genet 67:182–196

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hammer MF, Zegura SL (1996) The role of the Y chromosome in human evolutionary studies. Evol Anthropol 5:57–75

    Article  Google Scholar 

  • Hill CR, Duewer DL, Kline MC, Sprecher CJ, McLaren RS, Rabbach DR, Krenke BE, Ensenberger MG, Fulmer PM, Storts DR, Butler JM (2011) Concordance and population studies along with stutter and peak height ratio analysis for the PowerPlex® ESX 17 and ESI 17 systems. Forensic Sci Int Genet 5:269–275

    Article  CAS  PubMed  Google Scholar 

  • Hill CR, Duewer DL, Kline MC, Coble MD, Butler JM (2013) U.S. population data for 29 autosomal STR loci. Forensic Sci Int Genet 7:e82–e83

    Article  PubMed  Google Scholar 

  • Imaizumi K, Saitoh K, Sekiguchi K, Yoshino M (2002) Identification of fragmented bones based on anthropological and DNA analyses: case report. Leg Med 4:251–256

    Article  Google Scholar 

  • Jin HJ, Tyler-Smith C, Kim W (2009) The peopling of Korea revealed by analyses of mitochondrial DNA and Y-chromosomal markers. PLoS ONE 4:e4210

    Article  PubMed  PubMed Central  Google Scholar 

  • Kayser M, Caglià A, Corach D, Fretwell N, Gehrig C, Graziosi G, Heidorn F, Herrmann S, Herzog B, Hidding M, Honda K, Jobling M, Krawczak M, Leim K, Meuser S, Meyer E, Oesterreich W, Pandya A, Parson W, Penacino G, Perez-Lezaun A, Piccinini A, Prinz M, Schmitt C, Roewer L (1997) Evaluation of Y-chromosomal STRs: a multicenter study. Int J Legal Med 110:125–133

    Article  CAS  PubMed  Google Scholar 

  • Kraaijenbrink T, van Driem GL, Tshering of Gaselô K, de Knijff P (2007) Allele frequency distribution for 21 autosomal STR loci in Bhutan. Forensic Sci Int 170:68–72

    Article  CAS  PubMed  Google Scholar 

  • Krenke BE, Tereba A, Anderson SJ, Buel E, Culhane S, Finis CJ, Tomsey CS, Zachetti JM, Masibay A, Rabbach DR, Amiott EA, Sprecher CJ (2002) Validation of a 16-locus fluorescent multiplex system. J Forensic Sci 47:773–785

    Article  CAS  PubMed  Google Scholar 

  • Kwak KD, Jin HJ, Shin DJ, Kim JM, Roewer L, Krawczak M, Tyler-Smith C, Kim W (2005) Y-chromosomal STR haplotypes and their applications to forensic and population studies in east Asia. Int J Legal Med 119:195–201

    Article  PubMed  Google Scholar 

  • Lins AM, Micka KA, Sprecher CJ, Taylor JA, Bacher JW, Rabbach DR, Bever RA, Creacy SD, Schumm JW (1998) Development and population study of an eight-locus short tandem repeat (STR) multiplex system. J Forensic Sci 43:1168–1180

    Article  CAS  PubMed  Google Scholar 

  • Liu K, Muse SV (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128–2129

    Article  CAS  PubMed  Google Scholar 

  • Maruyama S, Minaguchi K, Takezaki N, Nambiar P (2008) Population data on 15 STR loci using AmpF/STR Indentifiler kit in a Malay population living in and around Kuala Lumpur, Malaysia. Leg Med 10:160–162

    Article  CAS  Google Scholar 

  • Novković T, Panić B, Banjac A, Dekić TK, Tomisić-Kosić I, Vucetić-Dragović A, Stamenković G, Blagojević J, Marjanović D, Pojskić N (2010) Genetic polymorphisms of 15 AmpFlSTR Identifiler loci in a Serbian population. Forensic Sci Int Genet 4:e149–e150

    Article  PubMed  Google Scholar 

  • Omran GA, Rutty GN, Jobling MA (2009) Genetic variation of 15 autosomal STR loci in Upper (Southern) Egyptians. Forensic Sci Int Genet 3:e39–e44

    Article  CAS  PubMed  Google Scholar 

  • Ota M, Droma Y, Basnyat B, Katsuyama Y, Asamura H, Sakai H, Fukuhsima H (2007) Allele frequencies for 15 STR loci in Tibetan populations from Nepal. Forensic Sci Int 169:234–238

    Article  CAS  PubMed  Google Scholar 

  • Park HC, Kim K, Nam Y, Park J, Lee J, Lee H, Kwon H, Jin H, Kim W, Kim W, Lim S (2016) Population genetic study for 24 STR loci and Y indel (GlobalFiler™ PCR Amplification kit and PowerPlex® Fusion system) in 1000 Korean individuals. Leg Med 21:53–57

    Article  CAS  Google Scholar 

  • Pfeiffer H, Forster P, Ortmann C, Brinkmann B (2001) The results of an mtDNA study of 1200 inhabitants of a German village in comparison to other Caucasian databases and its relevance for forensic casework. Int J Legal Med 114:169–172

    Article  CAS  PubMed  Google Scholar 

  • Piatek J, Jacewicz R, Ossowski A, Parafiniuk M, Berent J (2008) Population genetics of 15 autosomal STR loci in the population of Pomorze Zachodnie (NW Poland). Forensic Sci Int Genet 2:e41–e43

    Article  CAS  PubMed  Google Scholar 

  • Ramos-González B, Aguilar-Velázquez JA, de Lourdes Chávez-Briones M, Delgado-Chavarría JR, Alfaro-Lopez E, Rangel-Villalobos H (2016) Population data of 24 STRs in Mexican-Mestizo population from Monterrey, Nuevo Leon (Northeast, Mexico) based on PowerPlex® Fusion and GlobalFiler® kits. Forensic Sci Int Genet 21:e15–e17

    Article  PubMed  Google Scholar 

  • Rerkamnuaychoke B, Rinthachai T, Shotivaranon J, Jomsawat U, Siriboonpiputtana T, Chaiatchanarat K, Pasomsub E, Chantratita W (2006) Thai population data on 15 tetrameric STR loci-D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA. Forensic Sci Int 158:234–237

    Article  CAS  PubMed  Google Scholar 

  • Sadam M, Tasa G, Tiidla A, Lang A, Axelsson EP, Pajnič IZ (2015) Population data for 22 autosomal STR loci from Estonia. Int J Legal Med 129:1219–1220

    Article  CAS  PubMed  Google Scholar 

  • Schneider PM (2009) Expansion of the European standard set of DNA database loci—the current situation. Profiles DNA 12:6–7

    Google Scholar 

  • Smith BG, Lee B, Budowle B, Allen RW (2009) Population data for 15 STR loci (Identifiler kit) in a Filipino population. Leg Med 11:159–161

    Article  CAS  Google Scholar 

  • Sprecher C, Krenke B, Amiott B, Rabbach D, Grooms K (2000) The PowerPlex™ 16 System. Profiles DNA 4:3–6

    Google Scholar 

  • Tereba A (1999) Tools for analysis of population statistics. Profiles DNA 2:14–16

    Google Scholar 

  • Tie J, Wang X, Oxida S (2006) Genetic polymorphisms of 15 STR loci in a Japanese population. J Forensic Sci 51:188–189

    Article  CAS  PubMed  Google Scholar 

  • Tillmar AO, Bäckström G, Montelius K (2009) Genetic variation of 15 autosomal STR loci in a Somali population. Forensic Sci Int Genet 4:e19–e20

    Article  CAS  PubMed  Google Scholar 

  • Toscanini U, Moreno F, Pantoja-Astudillo JA, Morales EA, Bustos P, Salas A (2015) A reference frequency database of 15 autosomal STRs in Chile. Forensic Sci Int Genet 19:35–36

    Article  CAS  PubMed  Google Scholar 

  • Varga T, Keyser C, Beer Z, Penzes Z, Pamzsav H, Csete K, Ludes B (2003) Short tandem repeat data analysis in a Mongolian population. Leg Med 5(Suppl 1):S156–159

    Article  CAS  Google Scholar 

  • Weir BS (2007) The rarity of DNA profiles. Ann Appl Stat 1:358–370

    Article  PubMed  PubMed Central  Google Scholar 

  • Yuan L, Ou Y, Liao Q, Gui J, Bai X, Ge J, Ye J, Zhang L (2014) Population genetics analysis of 38 STR loci in the She population from Fujian Province of China. Leg Med 16:314–318

    Article  CAS  Google Scholar 

  • Zha L, Liu Y, Guo Y, Li J, Wang K, Geng K, Liao Q, Liu J, Chen H, Cai J (2014) Genetic polymorphism of 21 non-CODIS STR loci in the Chinese Mongolian ethnic minority. Forensic Sci Int Genet 9:e32–e33

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

We are grateful to all the volunteers for providing DNA samples in Mongolia. Technical assistance for the DNA preparation and PCR amplification of the sample in this study by Sun Yoon, A-Young Sim, Ji-Eun Lee, and Eun-Bi Shin was also greatly appreciated. This research was supported by a fund (NFS2015DNA06/NFS2016DNA04) from the Forensic Research Program of the National Forensic Service, South Korea. The National Institute of Forensic Science, Ulaanbaatar, Mongolia supports Ganbold Suren. This work is also supported in part by DANKOOK ChemBio Specialization for the Creative Korea-II (2016).

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Authors and Affiliations

  1. Department of Biological Sciences, Dankook University, Cheonan, 31116, Republic of Korea

    Eun-Ji Choi, Ji-Ae Kim, So-Yeon Kim, Hye-Min Kim & Wook Kim

  2. Forensic DNA Division, National Forensic Service, Wonju, 26460, Republic of Korea

    Ki-Won Park, Yang-Han Lee & Youn-Hyoung Nam

  3. Department of Scientific Analysis, National Institute of Forensic Science, Ulaanbaatar, 46611, Mongolia

    Ganbold Suren & Uyanga Ganbold

  4. Department of Neurology, University of California San Francisco, San Francisco, CA, 94158, USA

    Kicheol Kim

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  1. Eun-Ji Choi
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  2. Ki-Won Park
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  5. Ganbold Suren
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  9. Hye-Min Kim
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Correspondence to Wook Kim.

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The study was approved by the Ethics Committee and Institutional Review Board of Dankook University, Republic of Korea.

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Choi, EJ., Park, KW., Lee, YH. et al. Forensic and population genetic analyses of the GlobalFiler STR loci in the Mongolian population. Genes Genom 39, 423–431 (2017). https://doi.org/10.1007/s13258-016-0511-6

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