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Transcriptome sequencing and analysis of sweet osmanthus (Osmanthus fragrans Lour.)

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Abstract

Osmanthus fragrans is a woody, evergreen species of shrubs and small trees that is extensively planted in sub-tropical and temperate climates as an ornamental plant in gardens and for its health benefits. The flower color ranges from ivory to orange to pink among different varieties and even color difference during the whole blossom in the sweet osmanthus. Sweet osmanthus is widely cultivated throughout China and other countries due to its prominent fragrance, colorful flowers, and medicinal properties. However, the scanty genomic resources in the Olea family have greatly hindered further exploration of its genetic mechanism on these economically important traits. In this study, transcriptome sequencing of O. fragrans was performed using the Illumina HighSeqTM2000 sequencing platform. Next generation sequencing (NGS) of the transcriptome of O. fragrans produced 31.7G of clean bases (211,266,818 clean reads) that were assembled into 256,774 transcripts and 117,595 unigenes. Of them, 197 and 237 candidate genes involved in fragrance and pigment biosynthesis respectively were identified based on function annotation. Meanwhile, 1 unnamed protein and 468 functional unknown genes were also identified. Furthermore, mRNA sequencing expression profiling of O. fragrans were compared to previous genes’. In summary, this comprehensive transcriptome dataset allows the identification of genes associated with several major metabolic pathways and provides a useful public information platform for further functional genomic studiesin O. fragrans Lour.

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Abbreviations

GGPP:

Geranylgeranyl pyrophosphate

PSY:

Phytoene synthase

PDS:

15-Cis-phytoene desaturase

ZDE:

Zeta-carotene desaturase

PLYISO:

Prolycopene isomerase

CRTISO:

Carotenoids isomerase

β-LCY(LCYb):

β-lycopene cyclase

εLCY(LCYe):

ε-lycopene cyclase

ΒOH:

beta-carotene 3-hydroxylase

ZE:

Zeaxanthin epoxidase

VDE:

Violaxanthin de-epoxidase

CCS:

Capsanthin/capsorubin synthase

NS:

Neoxanthin synthase

NCED:

9-Cis-epoxycarotenoid dioxygenase

XDH:

Xanthoxin dehydrogenase

AAO:

Abscisic -aldehyde oxidase

AAH:

(+)-Abscisic acid 8′-hydroxylase

CYP73A:

Trans-cinnamate4-monooxygenase

CHS:

Chalcone synthase

CHI:

Chalcone isomerase

CYP93A:

Coumaroylquinate (coumaroyl shikimate) 3′-monooxygenase

FDO:

Naringenin 3-dioxygenase

HID:

2-Hydroxyisoflavanone dehydratase

FMO:

Flavonoid 3′-monooxygenase

LAD:

Leucoanthocyanidin dioxygenase

DFR:

Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase

ANS:

Anthocyanidin synthase

AGT:

Anthocyanidin 3-O-glucosyltransferase

ANR:

Anthocyanidin reductase

FLS:

Flavonol synthase

FMT:

Flavonol 3-O-methyltransferase

CMT:

Caffeoyl-CoAO-methyltransferase

SHCT:

ShikimateO-hydroxycinnamoyltransferase

CMO:

Coumaroylquinate (coumaroylshikimate) 3′-monooxygenase

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Acknowledgments

We appreciate the technical support for Illumina sequencing and initial data analysis from Novogene Bioinformatics Institute Beijing, China. We are grateful to Zong-Ming Cheng from University of Tennessee, Knoxville, for comments on the manuscript. We thank the anonymous referees and the editor for their comments and suggestions that helped improve the manuscript. We also thank the assistance provided by Yali Wang, Chunjun Wang, Tingting Shi, Ping Wang and Min Ling in preparing sweet osmanthus flowers. This study was supported by the Agriculture-Forestry Plant Germplasm Resources Exploration, Innovation and Utilization Project of the National Twelve-Five Science and Technology Support Program (2013BA001B06), and the Advantages Discipline Construction Engineering Project of Colleges and Universities in the Jiangsu province.

Conflict of interest

The authors declare that they have no competing interests.

Author information

Authors and Affiliations

  1. College of Landscape Architecture of Nanjing Forestry University, No. 159 Longpan Road, Nanjing, 210037, Jiangsu, China

    Hong Na Mu, Liang Gui Wang, Xiu Lian Yang & Chen Xu

  2. State Key Laboratory of Forestry Genetics Breeding and Gene Engineering of Nanjing Forestry University, No. 159 Longpan Road, Nanjing, 210037, Jiangsu, China

    Huo Gen Li

  3. College of Horticulture and Gardening, Yangtze University, No. 88 Jingmi Road, Jingzhou, 434025, Hubei, China

    Tao Ze Sun

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  1. Hong Na Mu
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  2. Huo Gen Li
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Correspondence to Liang Gui Wang.

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Mu, H.N., Li, H.G., Wang, L.G. et al. Transcriptome sequencing and analysis of sweet osmanthus (Osmanthus fragrans Lour.). Genes Genom 36, 777–788 (2014). https://doi.org/10.1007/s13258-014-0212-y

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