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Development of expressed sequence tag derived-simple sequence repeats in the genus Lilium

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Abstract

Although lily is the second largest flower crop in cutting flower commodity, only six simple sequence repeats SSRs have been reported. Thus, we developed expressed sequence tag derived-SSRs (EST-SSRs) for the Lilium genus. Among 2,235 unique ESTs, 754 ESTs contained SSR motifs, among which 165 ESTs were amenable to primer design. Among these 165 EST-SSRs, 131 EST-SSRs showed amplification in at least one Lilium species, and 76 EST-SSRs showed amplification in at least nine species. Of the 76 EST-SSRs, 47 showed amplification in all Lilium species analyzed. Using 10 breeding lines, we selected 21 EST-SSRs that had the highest number of alleles and polymorphism information content. The polymorphism information content values of these selected EST-SSRs ranged from 0.49 to 0.94 with an average of 0.76, which are higher than other plant species. The phylogenetic dendrogram derived from the amplification profiles of the 21 high polymorphic EST-SSRs was congruent with the genetic background of the 84 selected lily accessions and hybrids, which are available in commerce. Thus, the developed EST-SSRs will be very useful in germplasm management, genetic diversity analysis, cultivar finger printing, and molecular breeding in the lily.

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Authors and Affiliations

  1. Department of Molecular Biosciences, BK21 Training Team, Kangwon National University, Chuncheon, 200-701, Korea

    Sung-Il Lee, Jae-Han Son & Nam-Soo Kim

  2. Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, 200-701, Korea

    Kyong-Cheul Park & Nam-Soo Kim

  3. Oriental Bio-Herb Research Institute, Kangwon National University, Chuncheon, 200-701, Korea

    Ye-Su Song

  4. US Department of Agriculture-Agricultural Research Service, Western Regional Plant Introduction Station, Washington State University, 59 Johnson Hall, Pullman, WA, 99164, USA

    Soon-Jae Kwon

  5. Bio-crop Development Division, Department of Agricultural Bio-resources, National Academy of Agricultural Science, RDA, Suwon, 441-701, Korea

    Jong-Kuk Na

  6. Department of Horticulture, Kangwon National University, Chuncheon, 200-701, Korea

    Jong-Hwa Kim

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  1. Sung-Il Lee
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  2. Kyong-Cheul Park
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  3. Ye-Su Song
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  4. Jae-Han Son
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  7. Jong-Hwa Kim
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  8. Nam-Soo Kim
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Correspondence to Jong-Hwa Kim or Nam-Soo Kim.

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S.-I. Lee, K.-C. Park, and Y.-S. Song contributed equally to this work.

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Lee, SI., Park, KC., Song, YS. et al. Development of expressed sequence tag derived-simple sequence repeats in the genus Lilium . Genes Genom 33, 727–733 (2011). https://doi.org/10.1007/s13258-011-0203-1

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  • DOI: https://doi.org/10.1007/s13258-011-0203-1

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