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Characterization of Imcrop, a Mutator-like MITE family in the rice genome

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Abstract

Sequence comparisons of ammonium transporter 1–2 genes (OsAMT1-2) in different rice accessions revealed a MITE insertion in the upstream region of the gene. The 391-bp MITE, classified as a Mutator superfamily member and named Imcrop, included terminal inverted repeat (TIR) and 9-bp target site duplication (TSD) sequences. We identified 151 Imcrop elements dispersed on 12 chromosomes of the japonica reference genome. Of these, 12.6% were found in genic regions and 33.1% were located within 1.5 kb of annotated rice genes. We constructed comparative insertion maps with 111 and 102 intact Imcrop elements in the japonica and indica reference genomes, respectively. The Imcrop elements showed relatively even distribution across all chromosomes although their frequency was higher on chromosomes 1, 3, and 4 in both genomes. Seventy seven Imcrop elements were detected in both subspecies, whereas 34 and 25 insertions were found only in the japonica or indica genome, respectively. We compared insertion polymorphisms of 19 Imcrop elements found inside genes in 48 Korean rice cultivars, consisting of 42 japonica and six Tongil-types (indica-japonica cross). Thirteen insertions were common to all cultivars indicating these elements were present before indica-japonica divergence. The six other elements showed insertion polymorphisms among accessions, showing their recent insertion history or no critical positive effect of their insertion on the rice genome.

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Authors and Affiliations

  1. Deptartment of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 151-921, Korea

    Young-Jun Mo & Tae-Jin Yang

  2. Department of Rice and Winter Cereal Crop, National Institute of Crop Science, Rural Development Administration, Iksan, 570-080, Korea

    Young-Jun Mo, Ki-Young Kim, Woon-Chul Shin, Gun-Mi Lee, Jong-Cheol Ko, Jeong-Kwon Nam, Bo-Kyeong Kim & Jae-Kwon Ko

  3. Arizona Genomics Institute, School of Plant Sciences, BIO5 Institute, University of Arizona, Tucson, AZ, 85721, USA

    Yeisoo Yu

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  1. Young-Jun Mo
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  2. Ki-Young Kim
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  3. Woon-Chul Shin
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Correspondence to Tae-Jin Yang.

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Mo, YJ., Kim, KY., Shin, WC. et al. Characterization of Imcrop, a Mutator-like MITE family in the rice genome. Genes Genom 34, 189–198 (2012). https://doi.org/10.1007/s13258-011-0193-z

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  • DOI: https://doi.org/10.1007/s13258-011-0193-z

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