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Molecular identification of Ammodytes (PISCES, Ammodytidae) larvae, with ontogenetic evidence on separating populations

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Abstract

A total of ten larval Ammodytes were collected from the Yellow (5 specimens) and Bering Seas (5), and a 638 base pair sequence from their mitochondrial DNA COI genes was analyzed for species identification. Sequences were compared with those of adult Ammodytes personatus from the East (4), the Yellow (2) and the East China Seas (2), and Japan (3). Average genetic distance was 0.064 between the Yellow Sea larvae and the Bering Sea larvae, but was 0.0043 between the Yellow Sea larvae and all the adult A. personatus except two individuals from the East Sea. NJ-tree showed that five Yellow Sea larvae were closely clustered with adult A. personatus, except for two individuals. The five Bering Sea larvae were located distantly from adult A. personatus, and are thought to be A. hexapterus. We found morphological differences among two populations of A. personatus larvae and A. hexapterus larvae in morphometric characters and pigmentation. Our results indicated that the East Sea population of A. personatus is closer to A. hexapterus than to the Yellow Sea population of A. personatus, suggesting the East Sea population may be part of separate species.

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References

  • Cheng Q and Zheng B (1987) Systematic Synopsis of Chinese Fish.Vol. 1. Science Press, Beijing.

    Google Scholar 

  • Chun CI (1974) Biological studies on the sand-eel, Ammodytes personatus Girard. Bull. Kor. Fish. Soc. 7: 215–220.

    Google Scholar 

  • Chun CI (1979) Variation in the vertebral number of sand-eel, Ammodytes personatus Girard. Bull. Kor. Soc. Fish. Tech. 15: 1–3.

    Google Scholar 

  • Cohen DM (1984) Ontogeny, Systematics, and Phylogeny. In Ontogeny and Systematics of Fishes, H.G. Moser et al., eds., Amer. Soc. Ichthyol. Herp. pp. 7–11.

  • FishBase (2008) FishBase. http://www.fishbase.org/home.htm. Accessed in May 2008.

  • Fukui A, Tsuchiya T, Sezaki K and Watabe S (2008) Pelagic eggs and larvae of Coryphaenoides marginatus (Gadiformes: Macrouridae) collected from Suruga Bay, Japan. Ichthyol. Res. 55: 284–293.

    Article  Google Scholar 

  • Hatooka K (2002) Ammodytidae. In Fishes of Japan with Pictorial Keys to the Species, T. Nakabo, ed., Tokai Univ. Press, Tokyo, p. 1974.

    Google Scholar 

  • Heemstra PC and Parin NV (1986) Exocoetidae, In Smiths’ Sea Fishes, M.M. Smith and P.C. Heemstra, eds., CTP Book Printers, Cape, pp. 391–396.

    Google Scholar 

  • Hubert N, Delrieu-Trottin E, Irisson JO, Meyer C and Planes S (2010) Identifying coral reef fish larvae through DNA barcoding: A test case with the families Acanthuridae and Holocentridae. Mol. Phylogen. Evol. 55: 1195–1203.

    Article  CAS  Google Scholar 

  • Hyde JR, Lynn E, Humphreys R, Musyl M, West AP and Vetter R (2005) Shipboard identification of fish eggs and larvae by multiplex PCR, and description of fertilized eggs of blue marlin, shortbill spearfish, and wahoo. Mar. Eco. Prog. Ser. 286: 269–277.

    Article  CAS  Google Scholar 

  • Ida H, Sirimonataporn P and Monkolprasit S (1994) Comparative morphology of the fishes of the family Ammodytidae, with a description of two new genera and two new species. Zool. Stud. 33: 251–277.

    Google Scholar 

  • Johnson D (2005) Ontogeny and phylogenetic systematics: An old frontier, waiting. The abstract of the 7th Indo-Pacific Fish Conference. Taipei.

  • Kang CB (2000) Taxonomical studies on the genus Lateolabrax (Pisces, Perciformes) from the Korean waters. PhD Thesis. Pukyong Nat’l. Univ., Korea.

    Google Scholar 

  • Kim BJ, Kim S, Seo HS and Oh J (2008) Identification of Albula sp. (Albulidae: Albuliformes) leptocephalus collected from the southern coastal waters of Korea using cytochrome b DNA sequences. Oce. Sci. J. 43: 101–106.

    Article  Google Scholar 

  • Kim IS, Choi Y, Lee CL, Lee YJ, Kim BJ and Kim JH (2005) Illustrated Book of Korean Fishes. Kyohak, Seoul, 615 pp.

    Google Scholar 

  • Kim JK (2009) Diversity and conservation of Korean marine fishes. Kor. J. Ichthyol. 21: 52–62.

    Google Scholar 

  • Kim JK, Doiuchi R and Nakabo T (2006) Molecular and morphological differences between two geographic populations of Salanx ariakensis (Salangidae) from Korea. Ichthyol. Res. 53: 52–62.

    Article  Google Scholar 

  • Kim JK, Park JH, Kim YS, Kim YH, Hwang HJ, Hwang SJ, Lee SI and Kim TI (2008) Morphological differences among four geographic populations of Ammodytes personatus (Ammodytidae). J. Ichthyol. 48: 904–910.

    Article  Google Scholar 

  • Kim JK, Park JY and Kim YS (2006) Genetic diversity, relationship and demographic history of three geographic populations of Ammodytes personatus (Ammodytidae) from Korea inferred from mitochondrial DNA control region and 16S rRNA. Kor. J. Genet. 28: 343–351.

    CAS  Google Scholar 

  • Kim WJ, Kim KK, Han HS, Nam BH, Kim YO, Kong HJ, Noh JK and Yoon M (2010) Population structure of the olive flounder (Paralichthys olivaceus) in Korea inferred from microsatellite marker analysis. J. Fish Biol. 76: 1958–1971.

    Article  PubMed  Google Scholar 

  • Kim YH, Kang YJ and Ryu DG (1999) Growth of Ammodytes personatus in Korean Waters 1. Daily growth increment, early growth and spawning time in juvenile stage. J. Kor. Fish. Soc. 32: 550–555.

    Google Scholar 

  • Kimura M (1980) A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 116–120.

    Article  Google Scholar 

  • Kumar S, Tamura K and Nei M (1993) Guidelines for choosing distance measures. In MEGA: molecular evolutionary genetics analysis, version 1.01. University Park, Pennsylvania, pp. 27–29.

  • Lee HW (2007) Fishery biology of the sandfish, Arctoscopus japonicus in the East Sea of Korea. PhD Thesis. Pukyong Nat’l. Univ., Korea.

    Google Scholar 

  • Lindstrom DP (1999) Molecular species identification of newly hatched Hawaiian amphidromous gobioid larvae. Mar. Biotech. 1: 167–174.

    Article  CAS  Google Scholar 

  • Matarese AC, Kendall AW, Blood DM and Vinter BM (1989) Laboratory guide to early life history stages of northeast Pacific fishes. NOAA Tech. Rep. NMFS 80, 625 pp.

  • Nizinski MS, Collett BB and Washington BB (1990) Separation of two species of sand lance, Ammodytes americanus and A. dubius, in the western north Atlantic. Fish. Bull. 88: 241–255.

    Google Scholar 

  • Okamoto H (1989) A genetic comparison of sympatric populations of sand lance (genus Ammodytes) from the region east of Cape Soya, Japan (1). Can. J. Fish. Aqu. Sci. 46: 1945–1951.

    Article  Google Scholar 

  • Okamoto H, Sato H and Sjhhimazaki K (1989) Comparison of reproductive cycle between two genetically distinctive groups of sand lance (Genus Ammodytes) from northern Hokkaido. Nippon Suisan Gakkaishi 55: 1935–1940.

    Google Scholar 

  • Okamoto H, Yamazaki F and Mishima S (1988) Genetic divergence among sand-lance Ammodytes personatus populations in Japan. Nippon Suisan Gakkaishi 54: 1297–1304.

    Google Scholar 

  • Okiyama M (1988) An Atlas of the Early Stage Fishes in Japan. Tokai University Press, Tokyo, 1154 pp.

    Google Scholar 

  • Paine MA, McDowell JR and Graves JE (2008) ug Specific identification using COI sequence analysis of scombrid larvae collected off the Kona coast of Hawaii Island. Ichthyol. Res. 55: 7–16.

    Article  Google Scholar 

  • Parine NV (1996) On the species composition of flying fishes (Exocoetidae) in the west-central part of tropical Pacific. J. Ichthyol. 36: 357–364.

    Google Scholar 

  • Robards MD, Rose GA and Piatt JF (2002) Growth and abundance of Pacific sand lance, Ammodytes hexapterus, under differing oceanographic regimes. Environ. Biol. Fishes 64: 429–441.

    Article  Google Scholar 

  • Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB and Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239: 487–491.

    Article  CAS  PubMed  Google Scholar 

  • Saitou N and Nei M (1987) The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4: 406–425.

    CAS  PubMed  Google Scholar 

  • Taylor CA and Watson W (2004) Utility of larval pigmentation to identify nearshore rockfishes of the Sebastes subgenus Pteropodus from southern California. CalCOFI. Report 45: 113–117.

    Google Scholar 

  • Vandersea MW, Litaker RW, Marancik KE, Hare JA, Walsh HJ, Lem S, West MA, Wyanski DM, Laban EH and Tester PA (2008) Identification of larval sea basses (Centropristis spp.) using ribosomal DNA-specific molecular assays. Fish. Bull. 106: 183–193.

    Google Scholar 

  • Victor BC, Hanner R, Shivji M, Hyde J and Caldow C (2009) Identification of the larval and juvenile stages of the cubera snapper, Lutjanus cyanopterus, using DNA barcoding. Zootaxa 2215: 24–36.

    Google Scholar 

  • Ward RD, Zemlak TS, Innes BH, Last PR and Hebert PDN (2005) Barcoding Australia’s fish species. Philosophical Transactions of the Royal Society B 360: 1847–1857.

    Article  CAS  Google Scholar 

  • Watrous LE and Wheeler QD (1981) The outgroup comparison method of character analysis. Syst. Zool. 30: 1–11.

    Article  Google Scholar 

  • White BN, Lavenberg RJ and McGowen GE (1984) Atheriniformes: Development and relationships. In Ontogeny and Systematics of Fishes. Special Publication 1, H.G. Moser et al., eds., Amer. Soc. Ichthyol. Herp. pp. 355–362.

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Authors and Affiliations

  1. Department of Marine Biology, Pukyong National University, 599-1, Daeyeon 3-dong, Nam-gu, Busan, 608-737, Koreae

    Jin Koo Kim

  2. Southwest Fisheries Science Center, National Marine Fisheries Service, NOAA, La Jolla Shores Drive, La Jolla, California, 92037, USA

    William Watson, John Hyde & Nancy Lo

  3. Southwest Sea Fisheries Research Institute, National Fisheries Research and Development Institute, Yosu, 556-823, Korea

    Jin Young Kim

  4. Marine Living Resources Research Division, Korea Ocean Research and Development Institute, Ansan, 425-600, Korea

    Sung Kim

  5. Research and Development Planning Department, National Fisheries Research and Development Institute, Busan, 619-902, Korea

    Young Seop Kim

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  1. Jin Koo Kim
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  2. William Watson
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  3. John Hyde
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  4. Nancy Lo
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  5. Jin Young Kim
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  6. Sung Kim
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  7. Young Seop Kim
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Correspondence to Young Seop Kim.

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Kim, J.K., Watson, W., Hyde, J. et al. Molecular identification of Ammodytes (PISCES, Ammodytidae) larvae, with ontogenetic evidence on separating populations. Genes Genom 32, 437–445 (2010). https://doi.org/10.1007/s13258-010-0017-6

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  • DOI: https://doi.org/10.1007/s13258-010-0017-6

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